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Influence of T-Bar on Calcium Concentration Impacting Release Probability
Goethe Univ Frankfurt, Goethe Ctr Sci Comp GCSC, Frankfurt, Germany..
KTH, Centres, Science for Life Laboratory, SciLifeLab.
Goethe Univ Frankfurt, Goethe Ctr Sci Comp GCSC, Frankfurt, Germany.;King Abdullah Univ Sci & Technol, Aoplied Math & Computat Sci Comp Elect & Math Sci, Thuwal, Saudi Arabia..
2022 (English)In: Frontiers in Computational Neuroscience, E-ISSN 1662-5188, Vol. 16, article id 855746Article in journal (Refereed) Published
Abstract [en]

The relation of form and function, namely the impact of the synaptic anatomy on calcium dynamics in the presynaptic bouton, is a major challenge of present (computational) neuroscience at a cellular level. The Drosophila larval neuromuscular junction (NMJ) is a simple model system, which allows studying basic effects in a rather simple way. This synapse harbors several special structures. In particular, in opposite to standard vertebrate synapses, the presynaptic boutons are rather large, and they have several presynaptic zones. In these zones, different types of anatomical structures are present. Some of the zones bear a so-called T-bar, a particular anatomical structure. The geometric form of the T-bar resembles the shape of the letter "T" or a table with one leg. When an action potential arises, calcium influx is triggered. The probability of vesicle docking and neurotransmitter release is superlinearly proportional to the concentration of calcium close to the vesicular release site. It is tempting to assume that the T-bar causes some sort of calcium accumulation and hence triggers a higher release probability and thus enhances neurotransmitter exocytosis. In order to study this influence in a quantitative manner, we constructed a typical T-bar geometry and compared the calcium concentration close to the active zones (AZs). We compared the case of synapses with and without T-bars. Indeed, we found a substantial influence of the T-bar structure on the presynaptic calcium concentrations close to the AZs, indicating that this anatomical structure increases vesicle release probability. Therefore, our study reveals how the T-bar zone implies a strong relation between form and function. Our study answers the question of experimental studies (namely "Wichmann and Sigrist, Journal of neurogenetics 2010") concerning the sense of the anatomical structure of the T-bar.

Place, publisher, year, edition, pages
Frontiers Media SA , 2022. Vol. 16, article id 855746
Keywords [en]
T-bar, Drosophila larval NMJ, VGCC, calcium influx, diffusion-reaction PDE model, calcium microdomain, vesicle release probability, UG4
National Category
Developmental Biology Cell Biology Physiology and Anatomy
Identifiers
URN: urn:nbn:se:kth:diva-317034DOI: 10.3389/fncom.2022.855746ISI: 000840326900001PubMedID: 35586479Scopus ID: 2-s2.0-85130257230OAI: oai:DiVA.org:kth-317034DiVA, id: diva2:1693216
Note

QC 20220906

Available from: 2022-09-06 Created: 2022-09-06 Last updated: 2025-02-10Bibliographically approved

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Roy, Ranjita Dutta

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