Microwave-assisted methacrylation of chitosan for 3D printable hydrogels in tissue engineeringShow others and affiliations
2022 (English)In: Materials Advances, E-ISSN 2633-5409, Vol. 3, no 1, p. 514-525Article in journal (Refereed) Published
Abstract [en]
Light processable natural polymers are highly attractive for 3D printing of biomedical hydrogels with defined geometries and sizes. However, functionalization with photo-curable groups, such as methacrylate or acrylate groups, is required. Here, we investigated a microwave-assisted process for methacrylation of chitosan to replace conventional methacrylation processes that can be time consuming and tedious. The microwave-assisted methacrylation reaction was optimized by varying the synthesis parameters such as the molar ratio of chitosan to the methacrylic agent, the launch and reaction times and process temperature. The optimized process was fast and efficient and allowed tuning of the degree of substitution and thereby the final hydrogel properties. The successful methacrylation and degree of substitution were verified by 1H NMR and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The influence of the degree of methacrylation on photo-rheology, mechanical stiffness, swelling degree and gel content was evaluated. Furthermore, favourable 3D printability, enzymatic degradability, biocompatibility, cell migration and proliferation were demonstrated giving promise for further applications in tissue engineering.
Place, publisher, year, edition, pages
Royal Society of Chemistry (RSC) , 2022. Vol. 3, no 1, p. 514-525
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:kth:diva-319161DOI: 10.1039/d1ma00765cISI: 000720966000001Scopus ID: 2-s2.0-85122872780OAI: oai:DiVA.org:kth-319161DiVA, id: diva2:1700353
Note
QC 20230615
2022-09-302022-09-302023-06-15Bibliographically approved