kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience. Clemson Univ, Dept Environm Engn & Earth Sci, Clemson, SC 29634 USA..ORCID iD: 0000-0002-2052-9214
Clemson Univ, Dept Environm Engn & Earth Sci, Clemson, SC 29634 USA.;Michigan State Univ, Global Alliance Rapid Diagnost, E Lancing, MI 48824 USA..
MIT, Dept Mech Engn, MIT Auto ID Labs, 77 Massachusetts Ave, Cambridge, MA 02139 USA.;Harvard Med Sch, Med Device MDPnP Interoperabil & Cybersecur Labs, Dept Anesthesiol, Biomed Engn Program,Massachusetts Gen Hosp, 65 Landsdowne St, Cambridge, MA 02139 USA..
Clemson Univ, Dept Environm Engn & Earth Sci, Clemson, SC 29634 USA.;Michigan State Univ, Global Alliance Rapid Diagnost, E Lancing, MI 48824 USA.;Clemson Univ, Agr Sci, 821 McMillan Rd, Clemson, SC 29631 USA..ORCID iD: 0000-0002-1662-7372
Show others and affiliations
2022 (English)In: Biosensors, ISSN 2079-6374, Vol. 12, no 11, article id 938Article in journal (Refereed) Published
Abstract [en]

Biolayer interferometry (BLI) is a well-established laboratory technique for studying biomolecular interactions important for applications such as drug development. Currently, there are interesting opportunities for expanding the use of BLI in other fields, including the development of rapid diagnostic tools. To date, there are no detailed frameworks for implementing BLI in target-recognition studies that are pivotal for developing point-of-need biosensors. Here, we attempt to bridge these domains by providing a framework that connects output(s) of molecular interaction studies with key performance indicators used in the development of point-of-need biosensors. First, we briefly review the governing theory for protein-ligand interactions, and we then summarize the approach for real-time kinetic quantification using various techniques. The 2020 PRISMA guideline was used for all governing theory reviews and meta-analyses. Using the information from the meta-analysis, we introduce an experimental framework for connecting outcomes from BLI experiments (K-D, k(on), k(off)) with electrochemical (capacitive) biosensor design. As a first step in the development of a larger framework, we specifically focus on mapping BLI outcomes to five biosensor key performance indicators (sensitivity, selectivity, response time, hysteresis, operating range). The applicability of our framework was demonstrated in a study of case based on published literature related to SARS-CoV-2 spike protein to show the development of a capacitive biosensor based on truncated angiotensin-converting enzyme 2 (ACE2) as the receptor. The case study focuses on non-specific binding and selectivity as research goals. The proposed framework proved to be an important first step toward modeling/simulation efforts that map molecular interactions to sensor design.

Place, publisher, year, edition, pages
MDPI AG , 2022. Vol. 12, no 11, article id 938
Keywords [en]
biosensor design, protein-protein interaction, molecular affinity, binding kinetics, analytical sensing, SARS-CoV-2
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-322012DOI: 10.3390/bios12110938ISI: 000883862400001PubMedID: 36354449Scopus ID: 2-s2.0-85141803899OAI: oai:DiVA.org:kth-322012DiVA, id: diva2:1714795
Note

QC 20221130

Available from: 2022-11-30 Created: 2022-11-30 Last updated: 2022-11-30Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Ullah, Sadia Fida

Search in DiVA

By author/editor
Ullah, Sadia FidaMcLamore, Eric
By organisation
Glycoscience
In the same journal
Biosensors
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 61 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf