This bachelor thesis aimed to identify and test novel inhibitors that could potentially reduce methane emissions in ruminant animals. Methane is a potent greenhouse gas that significantly contributes to global warming, and ruminant livestock are a major source of methane emissions. The research involved a literature study, a preliminary screen of chemicals to reduce methane production from rumen fluid, and a detailed analysis of selected chemicals for methane reduction from rumen fluid. The preliminary screen was conducted in vitro. Rumen fluid was acquired and used to screen the candidates in a 20-hour incubation. The overhead gas was then measured for methane using a mass spectrometer. The detailed analysis was similar in design but incubated rumen fluid for 48 hours and the overhead gas was analysed with a gas spectrometer. Gas production was also measured using a gas production recorder.

Seven compounds were selected for a preliminary screen of chemicals to reduce methane production from rumen fluid based on their applicability determined in the literature study, where their price, toxicity, and environmental impact were included. Out of the seven candidates, six showed substantial inhibition of methane production in the preliminary screen, with candidate X showing the greatest potential with a 98% reduction of methane production in the detailed analysis of selected chemicals for methane reduction from rumen fluid.

The study concluded that candidate X has the potential to be an applicable feed additive for ruminants, offering a promising approach for reducing methane emissions from livestock. Further research, including in vivo testing, is necessary to fully understand the effectiveness of candidate X and its potential impact on the environment and animal health.