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Characterization studies of aluminum phthalocyanine binding to antibodies from SKBR 3 cell line
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
KTH, School of Biotechnology (BIO), Molecular Biotechnology.
KTH, School of Biotechnology (BIO), Molecular Biotechnology.ORCID iD: 0000-0002-5391-600X
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0003-0578-4003
2008 (English)Report (Other academic)
Place, publisher, year, edition, pages
2008. no 45
Series
Trita-FYS, ISSN 0280-316X ; 2008:45
Keyword [en]
antibody, photocleavage, confocal microscopy, FRAP, CALI, photobleaching, photochemical reaction
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-10043OAI: oai:DiVA.org:kth-10043DiVA: diva2:202239
Note
QC 20101102Available from: 2009-03-09 Created: 2009-03-09 Last updated: 2010-11-02Bibliographically approved
In thesis
1. Photophysical and Chemical Approaches to Cellular Biophysics
Open this publication in new window or tab >>Photophysical and Chemical Approaches to Cellular Biophysics
2008 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

The central theme in this thesis is reversibility. Two main attempts has been made to approach reversibility in cellular systems from both chemical and physical points of view. Reversibility of immunolabeling of proteins on the cell surface has been adressed by development of new fluorescent substances optimized for CALI (Chromophore-Assisted Laser Inactivation of protein). Aluminum phthalocyanine (AlPc) is here identified to be a good candidate for a new generation of fluorophores for efficient hydroxyl radical generation. It is shown that cells can be reversibly labeled with antibody-AlPc conjugates. In experiments on living cells the AlPcs were not only active as classic fluorophores but also as photocatalytic substances with destaining properties. Reversibility of cell immobilization is also reported, where cells cultured in microstructures were immobilized and 3D supported using hydrogels. Hydrogel formulation and application was optimized to achieve a system where both viability and ease of use was satisfied. Gel reversibility was actualized with pH and enzyme treatment. The developped method offers the possibility of stop flow culturing cells in controlled and reusable 3D environments.

Place, publisher, year, edition, pages
Stockholm: KTH, 2008. v, 26 p.
Series
Trita-FYS, ISSN 0280-316X ; 2008:54
National Category
Biophysics
Identifiers
urn:nbn:se:kth:diva-10097 (URN)978-91-7412-200-5 (ISBN)
Presentation
2008-12-18, FA 32, AlbaNova Universitetscentrum, Roslagstullsbacken 21, Stockholm, 11:00 (English)
Opponent
Supervisors
Note
QC 20101102Available from: 2009-03-16 Created: 2009-03-16 Last updated: 2010-11-02Bibliographically approved

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Gräslund, TorbjörnBrismar, Hjalmar

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