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Improved enzymatic activity of Thermomyces lanuginosus lipaseimmobilized in a hydrophobic particulate mesoporous carrier
YKI, Inst Surface Chem, SE-11486 Stockholm, Sweden .
2010 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 343, no 1, 359-365 p.Article in journal (Refereed) Published
Abstract [en]

Lipase from Thermomyces lanuginosus has been immobilized within particulate mesoporous silica carriers, with either hydrophilic or hydrophobic supporting surfaces, produced by the newly developed emulsion and solvent evaporation (ESE) method. The Michaelis-Menten model was used to calculate the parameters related to the enzymatic activity of lipase i.e. the turnover number, k(cat), and the specific activity. The specific activity was improved by immobilization of lipase onto the hydrophobic support, compared to lipase immobilized onto the hydrophilic support and lipase free in solution. The enhanced enzymatic activity of lipase onto a hydrophobic support was attributed to interfacial activation of the Thermomyces lanuginosus lipase when it is attached to a hydrophobic surface and a reduced denaturation. Confocal scanning laster microscopy (CLSM) studies, of fluorescently tagged lipase, showed that leakage of the lipase from the mesoporous particles was limited to all initial period of only a few hours. Both the rate and the amount of lipase leached were reduced when the lipase was immobilized onto the hydrophobic support.

Place, publisher, year, edition, pages
2010. Vol. 343, no 1, 359-365 p.
Keyword [en]
Lipase; Mesoporous; CLSM; Particulate; Carries; Enzymatic activity; Supporting surfaces
National Category
Physical Chemistry
URN: urn:nbn:se:kth:diva-10105DOI: 10.1016/j.jcis.2009.11.014ISI: 000274548900050OAI: diva2:208656
Uppdaterad från manuskript till artikel: 20100811 QC 20100811Available from: 2009-03-19 Created: 2009-03-19 Last updated: 2012-03-19Bibliographically approved
In thesis
1. Mesostructured particulate silica materials with tunable pore size: Synthesis, characterization and applications
Open this publication in new window or tab >>Mesostructured particulate silica materials with tunable pore size: Synthesis, characterization and applications
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Colloidal assemblies of surfactants and polymers in aqueous solutions have been used by human mankind for hundreds of years and they are of great importance in many of our technological processes, such as fabrication of soap and papermaking. Less than two decades ago the idea of using colloidal assemblies as templates of inorganic materials was borne. A new population of materials, referred to as surfactant templated materials, took form. These materials showed extraordinary properties such as monodisperse pore size distribution, large surface areas and pore volumes.


The main focus of this thesis has been on synthesis and functionalisation of spherical mesostructured silica particulate materials. In the first part of the work, mesostructured materials with expanded pores have been produced using a well established aerosol-based method as well as the newly developed emulsion and solvent evaporation (ESE) method. Increase in pore size was realized through using Pluronic block copolymer F127 together with a swelling agent poly(propylene glycol) as template. The influence of the swelling agent on pore size expansion was shown to have a roughly linear relationship. Furthermore, the impact of synthesis parameters on internal and exterior morphology has been investigated. Accessibility of the internal pore space, as well as the external surface roughness were shown to be highly dependent on synthesis temperature. Additionally, a very interesting well ordered 3D closed packed (P63/mmc) material was produced using the ionic surfactant C16TAB as template in the ESE method.


In the second part of the thesis work, mesoporous spheres with large pore size, having either hydrophilic or hydrophobic surface properties, were used as carriers of an enzyme, lipase. The enzymatic activity of lipase was increased onto the hydrophobic surface, compared to lipase immobilized into the hydrophilic support as well as for lipase free in solution. This effect was probably due to a combination of enhanced hydrophobic interactions preventing denaturation of the enzyme and interfacial activation of the enzyme.  This study generated an inorganic carrier material that is a promising candidate for biocatalysis applications. Additionally, mesoporous spheres were used as carriers of a model drug, Ibuprofen, to study the effect of polyelectrolyte multilayers on release properties. However, these layers were shown impermeable independent on pH and the substance was only released from uncoated particles.

Place, publisher, year, edition, pages
Stockholm: KTH, 2009. 70 p.
TRITA-CHE-Report, ISSN 1654-1081 ; 2009:8
Mesoporous materials, aersol-based process, emulsion-based process, biocatalysis, sustained release, 3D hexagonal, 2D hexagonal, powder, spherical particles, liquid crystalline phases, AFM-porosimetry, Pluronic
National Category
Other Basic Medicine
urn:nbn:se:kth:diva-10089 (URN)978-91-7415-243-2 (ISBN)
Public defence
2009-04-03, E2, Lindstedsvägen 3, Stockholm, 10:00 (English)

QC 20100811

Available from: 2009-03-19 Created: 2009-03-11 Last updated: 2014-11-03Bibliographically approved

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Sörensen, Malin H.
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