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Bioaugmentation for enhanced denitrification in a labscale treatment system
KTH, School of Biotechnology (BIO), Environmental Microbiology (closed September 2009). (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology (closed September 2009). (Miljömikrobiologi)
2006 (English)In: Proceedings of the Second IASTED International Conference on Advanced Technology in the Environmental Field / [ed] Ubertini, L, ACTA Press, 2006, 63-67 p.Conference paper, Published paper (Refereed)
Abstract [en]

The effect of bioaugmentation was investigated in a predenitrification lab-scale wastewater treatment system. The aim was to investigate the difference between two approaches to bioaugmentation: one in which suspended overnight culture was used as inoculum and another where bacteria immobilized in 1% agar beads were used. Pure cultures of the denitrifying bacteria Comamonas denitrificans ATCC 700936T were used in the experiments. The effect of bioaugmentation on the system was monitored using fluorescent in situ hybridization (FISH) and denitrification activity tests. The bioaugmentation with suspended bacteria showed a rapid initial (4 days) increase in denitrification activity. After 8 days the activity declined to the level of the reference system and cells of C. denitrificans were no longer detectable. Augmentation with agar-embedded bacteria resulted in a small increase in activity and very few bacteria of C. denitrificans could be observed.

Place, publisher, year, edition, pages
ACTA Press, 2006. 63-67 p.
Keyword [en]
Bioaugmentation, denitrification, fluorescent in situ hybridization, immobilization, wastewater treatment, Comamonas denitrificans
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:kth:diva-10152ISI: 000236368300012Scopus ID: 2-s2.0-38049140883ISBN: 978-088986554-9 (print)OAI: oai:DiVA.org:kth-10152DiVA: diva2:209534
Conference
2nd IASTED International Conference on Advanced Technology in the Environmental Field Location: Lanzarote, Spain, Date: FEB 06-08, 2006
Note

QC 20100622

Available from: 2009-03-25 Created: 2009-03-25 Last updated: 2014-11-07Bibliographically approved
In thesis
1. Characterization of Bacterial Biofilms for Wastewater Treatment
Open this publication in new window or tab >>Characterization of Bacterial Biofilms for Wastewater Treatment
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Research performed at the Division of Environmental Microbiology has over the last years resulted in the isolation of possible bacterial key-organisms with efficient nutrient removal properties (Comamonas denitrificans, Brachymonas denitrificans, Aeromonas hydrophila). Effective use of these organisms for enhanced nutrient removal in wastewater treatment applications requires the strains to be retained, to proliferate and to maintain biological activity within theprocess. This can be achieved by immobilization of the organisms using an appropriate system.Two putative immobilization systems, agar entrapment and biofilm formation, wereassessed. Surface attached biofilm growth provided better results with respect to cell retention,proliferation and microbial activity than immobilization in agar beads. Thus, biofilm physiology was further characterized using simplified systems of single, dual or multi strain bacterial consortia containing the key-organisms as well as other wastewater treatment isolates. Mechanisms for initial adherence, biofilm formation over time, dynamics and characteristics of extracellular polymeric substances (EPS) and exopolysaccharides, nutrient removal activity as well as the effect of bacterial interactions were investigated. The results showed that all theassessed bacterial strains could form single strain biofilm providing that a suitable nutrientsupply was given. Production of EPS was found to be critical for biofilm development and both EPS and polysaccharide residue composition varied with bacterial strain, culture conditions and biofilm age. Denitrification and phosphorus removal activity of the keyorganisms was maintained in biofilm growth. Co-culturing of two or more strains resulted in both synergistic and antagonistic effects on biofilm formation as well as the microbial activitywithin the biofilm. Bacterial interactions also induced the synthesis of new polysaccharideswhich were not produced in pure strain biofilms.The complexity of single and mixed strain biofilm development and the implications of interactions on biofilm performance were underlined in this study. The data presented can be useful for modeling of biofilm systems, serve as a tool for selection of bacterial strain combinations to use for bioaugmentation/bioremediation or provide a base for further experiment design.

Place, publisher, year, edition, pages
Stockholm: Kungliga Tekniska Högskolan, 2009. viii, 63 p.
Series
Trita-BIO-Report, ISSN 1654-2312
Keyword
biofilm, extracellular polymeric substances, exopolysaccharides, interspecies interactions, wastewater treatment, denitrification, phosphorus removal
National Category
Microbiology
Identifiers
urn:nbn:se:kth:diva-10118 (URN)978-91-7415-255-5 (ISBN)
Public defence
2009-04-17, Hörsal K1, KTH, Teknikringen 56, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note
QC 20100622Available from: 2009-03-25 Created: 2009-03-23 Last updated: 2010-07-19Bibliographically approved

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