Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Biofilm formation and interactions of bacterial strains found in wastewater treatment systems
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
2008 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 283, no 1, 83-90 p.Article in journal (Refereed) Published
Abstract [en]

Biofilm formation and adherence properties of 13 bacterial strains commonly found in wastewater treatment systems were studied in pure and mixed cultures using a crystal violet microtiter plate assay. Four different culture media were used, wastewater, acetate medium, glucose medium and diluted nutrient broth. The medium composition strongly affected biofilm formation. All strains were able to form pure culture biofilms within 24 h in at least one of the tested culture media and three strains were able to form biofilm in all four culture media, namely Acinetobacter calcoaceticus ATCC 23055, Comamonas denitrificans 123 and Pseudomonas aeruginosa MBL 0199. The adherence properties assessed were initial adherence, cell surface hydrophobicity, and production of amyloid fibers and extracellular polymeric substances. The growth of dual-strain biofilms showed that five organisms formed biofilm with all 13 strains while seven formed no or only weak biofilm when cocultured. In dual-strain cultures, strains with different properties were able to complement each other, giving synergistic effects. Strongest biofilm formation was observed when a mixture of all 13 bacteria were grown together. These results on attachment and biofilm formation can serve as a tool for the design of tailored systems for the degradation of municipal and industrial wastewater.

Place, publisher, year, edition, pages
OXFORD: BLACKWELL PUBLISHING , 2008. Vol. 283, no 1, 83-90 p.
Keyword [en]
biofilm formation; FISH; initial adherence; wastewater treatment
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-10153DOI: 10.1111/j.1574-6968.2008.01149.xISI: 000255286100013Scopus ID: 2-s2.0-42649086030OAI: oai:DiVA.org:kth-10153DiVA: diva2:209551
Note
QC 20100622Available from: 2009-03-25 Created: 2009-03-25 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Characterization of Bacterial Biofilms for Wastewater Treatment
Open this publication in new window or tab >>Characterization of Bacterial Biofilms for Wastewater Treatment
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Research performed at the Division of Environmental Microbiology has over the last years resulted in the isolation of possible bacterial key-organisms with efficient nutrient removal properties (Comamonas denitrificans, Brachymonas denitrificans, Aeromonas hydrophila). Effective use of these organisms for enhanced nutrient removal in wastewater treatment applications requires the strains to be retained, to proliferate and to maintain biological activity within theprocess. This can be achieved by immobilization of the organisms using an appropriate system.Two putative immobilization systems, agar entrapment and biofilm formation, wereassessed. Surface attached biofilm growth provided better results with respect to cell retention,proliferation and microbial activity than immobilization in agar beads. Thus, biofilm physiology was further characterized using simplified systems of single, dual or multi strain bacterial consortia containing the key-organisms as well as other wastewater treatment isolates. Mechanisms for initial adherence, biofilm formation over time, dynamics and characteristics of extracellular polymeric substances (EPS) and exopolysaccharides, nutrient removal activity as well as the effect of bacterial interactions were investigated. The results showed that all theassessed bacterial strains could form single strain biofilm providing that a suitable nutrientsupply was given. Production of EPS was found to be critical for biofilm development and both EPS and polysaccharide residue composition varied with bacterial strain, culture conditions and biofilm age. Denitrification and phosphorus removal activity of the keyorganisms was maintained in biofilm growth. Co-culturing of two or more strains resulted in both synergistic and antagonistic effects on biofilm formation as well as the microbial activitywithin the biofilm. Bacterial interactions also induced the synthesis of new polysaccharideswhich were not produced in pure strain biofilms.The complexity of single and mixed strain biofilm development and the implications of interactions on biofilm performance were underlined in this study. The data presented can be useful for modeling of biofilm systems, serve as a tool for selection of bacterial strain combinations to use for bioaugmentation/bioremediation or provide a base for further experiment design.

Place, publisher, year, edition, pages
Stockholm: Kungliga Tekniska Högskolan, 2009. viii, 63 p.
Series
Trita-BIO-Report, ISSN 1654-2312
Keyword
biofilm, extracellular polymeric substances, exopolysaccharides, interspecies interactions, wastewater treatment, denitrification, phosphorus removal
National Category
Microbiology
Identifiers
urn:nbn:se:kth:diva-10118 (URN)978-91-7415-255-5 (ISBN)
Public defence
2009-04-17, Hörsal K1, KTH, Teknikringen 56, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note
QC 20100622Available from: 2009-03-25 Created: 2009-03-23 Last updated: 2010-07-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopushttp://www3.interscience.wiley.com/journal/119404434/abstract?CRETRY=1&SRETRY=0

Search in DiVA

By author/editor
Andersson, SofiaKuttuva Rajarao, GunaratnaLand, Carl JohanDalhammar, Gunnel
By organisation
Environmental Microbiology
In the same journal
FEMS Microbiology Letters
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 136 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf