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Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
KTH, School of Biotechnology (BIO), Environmental Microbiology. (Miljömikrobiologi)
2009 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 82, no 3, 535-543 p.Article in journal (Refereed) Published
Abstract [en]

Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment (WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (3-37%), nucleic acids (9-50%), and carbohydrates (3-21%). The extracellular DNA was found to be important for initial biofilm formation since biofilm, but not planktonic growth, was inhibited in the presence of DNase. The polysaccharide fraction appeared to consist of at least two distinct polymers, one branched fraction (A) made up of glucose and mannose with a molecular weight around 100 kDa. The other fraction (B) was larger and consisted of ribose, mannose, glucose, rhamnose, arabinose, galactose, and N-acetylglucosamine. Fraction B polysaccharides were mainly found in capsular EPS which was the dominant type in biofilms and agar-grown colonies. Fraction A was abundant in the released EPS, the dominant type in planktonic cultures. Biofilm and agar-grown EPS displayed similar overall properties while planktonic EPS showed clear compositional disparity. This study presents results on the physiology of a key WWT organism, which may be useful in the future development of improved biofilm techniques for WWT purposes.

Place, publisher, year, edition, pages
2009. Vol. 82, no 3, 535-543 p.
Keyword [en]
Biofilm; Comamonas denitrificans; GC-MS; Extracellular polymeric substances; HPAEC; Polysaccharides
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-10155DOI: 10.1007/s00253-008-1817-3ISI: 000263484600014Scopus ID: 2-s2.0-60549106192OAI: oai:DiVA.org:kth-10155DiVA: diva2:209559
Note
QC 20100622Available from: 2009-03-25 Created: 2009-03-25 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Characterization of Bacterial Biofilms for Wastewater Treatment
Open this publication in new window or tab >>Characterization of Bacterial Biofilms for Wastewater Treatment
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Research performed at the Division of Environmental Microbiology has over the last years resulted in the isolation of possible bacterial key-organisms with efficient nutrient removal properties (Comamonas denitrificans, Brachymonas denitrificans, Aeromonas hydrophila). Effective use of these organisms for enhanced nutrient removal in wastewater treatment applications requires the strains to be retained, to proliferate and to maintain biological activity within theprocess. This can be achieved by immobilization of the organisms using an appropriate system.Two putative immobilization systems, agar entrapment and biofilm formation, wereassessed. Surface attached biofilm growth provided better results with respect to cell retention,proliferation and microbial activity than immobilization in agar beads. Thus, biofilm physiology was further characterized using simplified systems of single, dual or multi strain bacterial consortia containing the key-organisms as well as other wastewater treatment isolates. Mechanisms for initial adherence, biofilm formation over time, dynamics and characteristics of extracellular polymeric substances (EPS) and exopolysaccharides, nutrient removal activity as well as the effect of bacterial interactions were investigated. The results showed that all theassessed bacterial strains could form single strain biofilm providing that a suitable nutrientsupply was given. Production of EPS was found to be critical for biofilm development and both EPS and polysaccharide residue composition varied with bacterial strain, culture conditions and biofilm age. Denitrification and phosphorus removal activity of the keyorganisms was maintained in biofilm growth. Co-culturing of two or more strains resulted in both synergistic and antagonistic effects on biofilm formation as well as the microbial activitywithin the biofilm. Bacterial interactions also induced the synthesis of new polysaccharideswhich were not produced in pure strain biofilms.The complexity of single and mixed strain biofilm development and the implications of interactions on biofilm performance were underlined in this study. The data presented can be useful for modeling of biofilm systems, serve as a tool for selection of bacterial strain combinations to use for bioaugmentation/bioremediation or provide a base for further experiment design.

Place, publisher, year, edition, pages
Stockholm: Kungliga Tekniska Högskolan, 2009. viii, 63 p.
Series
Trita-BIO-Report, ISSN 1654-2312
Keyword
biofilm, extracellular polymeric substances, exopolysaccharides, interspecies interactions, wastewater treatment, denitrification, phosphorus removal
National Category
Microbiology
Identifiers
urn:nbn:se:kth:diva-10118 (URN)978-91-7415-255-5 (ISBN)
Public defence
2009-04-17, Hörsal K1, KTH, Teknikringen 56, Stockholm, 10:00 (Swedish)
Opponent
Supervisors
Note
QC 20100622Available from: 2009-03-25 Created: 2009-03-23 Last updated: 2010-07-19Bibliographically approved

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