Modulation Filtering Enables Removal of Spikes in Fluorescence Correlation Spectroscopy Measurements without Affecting the Temporal Information
2009 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 113, no 25, 8752-8757 p.Article in journal (Refereed) Published
The appearance of intensity spikes in measurements is a common problem in fluorescence correlation spectroscopy (FCS) studies of biological samples. In this work, we present a new method for generating artifact-free correlation curves from fluorescence traces that have undergone spike removal. This method preserves the temporal information throughout the measurement and properly represents the correlation between events separated by removed spikes. The method was validated using experimental data. The proposed algorithm is demonstrated herein to be generally applicable, but it is particularly powerful for cases where spikes occur frequently.
Place, publisher, year, edition, pages
2009. Vol. 113, no 25, 8752-8757 p.
IdentifiersURN: urn:nbn:se:kth:diva-10238DOI: 10.1021/jp902538bISI: 000267205600045ScopusID: 2-s2.0-67649218418OAI: oai:DiVA.org:kth-10238DiVA: diva2:212171
QC 201008052009-04-212009-04-212010-12-06Bibliographically approved