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Flow-free transport of cells in microchannels by frequency-modulated ultrasound
KTH, School of Engineering Sciences (SCI), Applied Physics. (Biomedical & X-Ray Physics)ORCID iD: 0000-0002-4720-2756
KTH, School of Engineering Sciences (SCI), Applied Physics. (Cell Physics)
KTH, School of Engineering Sciences (SCI), Applied Physics. (Cell Physics)ORCID iD: 0000-0001-5178-7593
KTH, School of Engineering Sciences (SCI), Applied Physics. (Biomedical & X-Ray Physics)
2009 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 9, 833-837 p.Article in journal (Refereed) Published
Abstract [en]

We demonstrate flow-free transport of cells and particles by the use of frequency-modulated ultrasonic actuation of a microfluidic chip. Two different modulation schemes are combined: A rapid (1 kHz) linear frequency sweep around similar to 6.9 MHz is used for two-dimensional spatial stabilization of the force field over a 5 mm long inlet channel of constant cross section, and a slow (0.2-0.7 Hz) linear frequency sweep around similar to 2.6 MHz is used for flow-free ultrasonic transport and positioning of cells or particles. The method is used for controlling the motion and position of cells monitored with high-resolution optical microscopy, but can also be used more generally for improving the robustness and performance of ultrasonic manipulation micro-devices.

Place, publisher, year, edition, pages
2009. Vol. 9, 833-837 p.
Keyword [en]
manipulation; particles; chip; separation; channels
National Category
Condensed Matter Physics
Identifiers
URN: urn:nbn:se:kth:diva-10918DOI: 10.1039/B816675GISI: 000263847000012Scopus ID: 2-s2.0-61849133822OAI: oai:DiVA.org:kth-10918DiVA: diva2:231814
Note
QC 20100730Available from: 2009-08-18 Created: 2009-08-18 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Multidimensional Ultrasonic Standing Wave Manipulation in Microfluidic Chips
Open this publication in new window or tab >>Multidimensional Ultrasonic Standing Wave Manipulation in Microfluidic Chips
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The use of ultrasonic standing waves for contactless manipulation of microparticles in microfluidic systems is a field with potential to become a new standard tool in lab-on-chip systems. Compared to other contactless manipulation methods ultrasonic standing wave manipulation shows promises of gentle cell handling, low cost, and precise temperature control. The technology can be used both for batch handling, such as sorting and aggregation, and handling of single particles.

This doctoral Thesis presents multi-dimensional ultrasonic manipulation, i.e., manipulation in both two and three spatial dimensions as well as time-dependent manipulation of living cells and microbeads in microfluidic systems. The lab-on-chip structures used allow for high-quality optical microscopy, which is central to many bio-applications. It is demonstrated how the ultrasonic force fields can be spatially confined to predefined regions in the system, enabling sequential manipulation functions. Furthermore, it is shown how frequency-modulated signals can be used both for spatial stabilization of the force fields as well as for flow-free transport of particles in a microchannel. Design parameters of the chip-transducer systems employed are investigated experimentally as well as by numerical simulations. It is shown that three-dimensional resonances in the solid structure of the chip strongly influences the resonance shaping in the channel.

Place, publisher, year, edition, pages
Stockholm: KTH, 2009. ix, 83 p.
Series
Trita-FYS, ISSN 0280-316X ; 2009:44
National Category
Condensed Matter Physics
Identifiers
urn:nbn:se:kth:diva-10919 (URN)978-91-7415-398-9 (ISBN)
Public defence
2009-09-11, FD5, Roslagstullsbacken 21, Stockholm, 14:00 (English)
Opponent
Supervisors
Note
QC 20100730Available from: 2009-09-01 Created: 2009-08-18 Last updated: 2010-07-30Bibliographically approved

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Manneberg, OttoÖnfelt, Björn

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