Protein-Specific Analysis of Humoral Immune Responses in a Clinical Trial for Vaccines against Contagious Bovine Pleuropneumonia
2010 (English)In: Clinical and Laboratory Immunology, ISSN 1556-6811, E-ISSN 1556-679X, Vol. 17, no 5, 853-861 p.Article in journal (Refereed) Published
Specific humoral immune responses in a clinical trial on cattle for vaccines against contagious bovine pleuropneumonia (CBPP) were investigated. The trial included a subunit vaccine consisting of five recombinant putative variable surface proteins of the infectious agent Mycoplasma mycoides subspecies mycoides small colony type (M. mycoides SC) compared to the currently approved attenuated vaccine strain T1/44 and untreated controls. Humoral immune responses to 65 individual recombinant surface proteins of M. mycoides SC were monitored by a recently developed bead based array assay. Responses to the subunit vaccine components were found to be weak. Animals vaccinated with this vaccine were not protected and had CBPP lesions similar to the untreated controls. In correlating protein-specific humoral responses to T1/44 induced immunity, five proteins associated with a protective immune response were identified,namely LppQ and those of ORFs MSC_0271, MSC_0136, MSC_0079 and MSC_0431. The five proteins may be important candidates in the development of a novel subunit vaccine against CBPP.
Place, publisher, year, edition, pages
2010. Vol. 17, no 5, 853-861 p.
subsp mycoides sc, small colony type, lipoprotein lppq, genetic-characterization, cbpp, cattle, eradication, africa, strategies, infection
IdentifiersURN: urn:nbn:se:kth:diva-11107DOI: 10.1128/CVI.00019-10ISI: 000277243200023ScopusID: 2-s2.0-77951988744OAI: oai:DiVA.org:kth-11107DiVA: diva2:235815
Uppdaterad från manuskript 20100719
Tidigare titel: Protein-specific analysis of humoral immune responses in a CBPP vaccine trial
QC 201007192009-09-182009-09-182011-01-19Bibliographically approved