Visual DNA: Identification of DNA sequence variations by bead trapping
2007 (English)In: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 90, 741-745 p.Article in journal (Refereed) Published
In this paper we describe a method that uses the nearly covalent strength biotin-streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with ail ordinary mobile phone equipped with a built-in camera.
Place, publisher, year, edition, pages
2007. Vol. 90, 741-745 p.
imaging techniques; medical imaging; magnetics; microspheres; oligonucleotide array; sequence analysis; signal amplification assay
IdentifiersURN: urn:nbn:se:kth:diva-11218DOI: 10.1016/j.ygeno.2007.07.014ISI: 000251707500010OAI: oai:DiVA.org:kth-11218DiVA: diva2:242207
QC 201007132009-10-072009-10-072010-07-15Bibliographically approved