Analysis of Short Tandem Repeats by Parallel DNA Threading
2009 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 4, no 11, e7823- p.Article in journal (Refereed) Published
The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.
Place, publisher, year, edition, pages
2009. Vol. 4, no 11, e7823- p.
eukaryotic genomes; microsatellites; evolution
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-11292DOI: 10.1371/journal.pone.0007823ISI: 000271721900014ScopusID: 2-s2.0-70649103962OAI: oai:DiVA.org:kth-11292DiVA: diva2:272176
QC 201008192009-10-142009-10-142010-12-07Bibliographically approved