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Surface characteristics, copper release, and toxicity of nano- and micrometer-sized copper and copper(II) oxide particles: a cross-disciplinary study.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.
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2009 (English)In: Small (Weinheim an der Bergstrasse, Germany), ISSN 1613-6829, Vol. 5, no 3, 389-99 p.Article in journal (Refereed) Published
Abstract [en]

An interdisciplinary and multianalytical research effort is undertaken to assess the toxic aspects of thoroughly characterized nano- and micrometer-sized particles of oxidized metallic copper and copper(II) oxide in contact with cultivated lung cells, as well as copper release in relevant media. All particles, except micrometer-sized Cu, release more copper in serum-containing cell medium (supplemented Dulbecco's minimal essential medium) compared to identical exposures in phosphate-buffered saline. Sonication of particles for dispersion prior to exposure has a large effect on the initial copper release from Cu nanoparticles. A clear size-dependent effect is observed from both a copper release and a toxicity perspective. In agreement with greater released amounts of copper per quantity of particles from the nanometer-sized particles compared to the micrometer-sized particles, the nanometer particles cause a higher degree of DNA damage (single-strand breaks) and cause a significantly higher percentage of cell death compared to cytotoxicity induced by micrometer-sized particles. Cytotoxic effects related to the released copper fraction are found to be significantly lower than the effects related to particles. No DNA damage is induced by the released copper fraction.

Place, publisher, year, edition, pages
2009. Vol. 5, no 3, 389-99 p.
Keyword [en]
copper; cytotoxicity; DNA damage; nanoparticles; particle characterization
National Category
Physical Chemistry Other Chemistry Topics
Identifiers
URN: urn:nbn:se:kth:diva-11691DOI: 10.1002/smll.200801220ISI: 000263503700017PubMedID: 19148889Scopus ID: 2-s2.0-60849117522OAI: oai:DiVA.org:kth-11691DiVA: diva2:279512
Note

QC 20100803

Available from: 2009-12-03 Created: 2009-12-03 Last updated: 2016-05-25Bibliographically approved
In thesis
1. Metal Particles – Hazard or Risk? Elaboration and Implementation of a Research Strategy from a Surface and Corrosion Perspective
Open this publication in new window or tab >>Metal Particles – Hazard or Risk? Elaboration and Implementation of a Research Strategy from a Surface and Corrosion Perspective
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Do metal particles (including particles of pure metals, alloys, metal oxides and compounds) pose a hazard or risk to human health? In the light of this question, this thesis summarizes results from research conducted on metal particles, and describes the elaboration and implementation of an in vitro test methodology to study metal release from particles through corrosion and dissolution processes in synthetic biological media relevant for human exposure through inhalation/ingestion and dermal contact.

Bioaccessible metals are defined as the pool of released metals from particles that potentially could be made available for absorption by humans or other organisms. Studies of bioaccessible metals from different metal particles within this thesis have shown that the metal release process is influenced by material properties, particle specific properties, size distribution, surface area and morphology, as well as the chemistry of synthetic biological test media simulating various human exposure scenarios. The presence of metal particles in proximity to humans and the fact that metals can be released from particles to a varying extent is the hazard referred to in the title.

The bioavailable metal fraction of the released metals (the fraction available for uptake/absorption by humans through different exposure routes) is usually significantly smaller than the bioaccessible pool of released metals, and is largely related to the chemical form and state of oxidation of the released metals. Chemical speciation measurements of released chromium for instance revealed chromium to be complexed to its non-available form in simulated lung fluids. Such measurements provide an indirect measure of the potential risk for adverse health effects, when performed at relevant experimental conditions.

A more direct way to assess risks is to conduct toxicological in-vitro testing of metal particles, for instance on lung cell cultures relevant for human inhalation. Induced toxicity of metal particles on lung cells includes both the effect of the particles themselves and of the released metal fraction (including bioaccessible and bioavailable metals), the latter shown to be less predominant. The toxic response was clearly influenced by various experimental conditions such as sonication treatment of particles and the presence of serum proteins.

Thorough characterization of metal particles assessing parameters including chemical surface composition, degree of agglomeration in solution, size distribution, surface area and morphology was performed and discussed in relation to generated results of bioaccessibility, bioavailability and induced toxicity. One important conclusion was that neither the surface composition nor the bulk composition can be used to assess the extent of metals released from chromium-based alloy particles. These findings emphasize that information on physical-chemical properties and surface characteristics of particles is essential for an in-depth understanding of metal release processes and for further use and interpretation of bioaccessibility data to assess hazard and reduce any risks induced by human exposure to metal particles.

Place, publisher, year, edition, pages
Stockholm: KTH, 2009. xiv, 69 p.
Series
Trita-CHE-Report, ISSN 1654-1081 ; 2009:56
Keyword
ferro-chromium alloy, metal particles, bioaccessibility, chemical speciation, dermal contact, surface oxide, in-vitro testing, chemical speciation, cu, copper, comet assay, intracellular, ultrafine, in vitro, A549, cytotoxicity, DNA damage, nanoparticles, particle characterization, artificial sweat, nickel release, nickel powder particles, particle loadings, release kinetics, surface area, copper release, powder particles, synthetic body fluids, simulation of interstitial lung conditions, skin contact, metal release, stainless steel, particles, test method
National Category
Physical Chemistry Analytical Chemistry Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-11695 (URN)978-91-7415-472-6 (ISBN)
Public defence
2009-12-14, F3, Lindstedtsvägen 26, KTH, Stockholm, 10:00 (English)
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Supervisors
Note
QC 20100803Available from: 2009-12-04 Created: 2009-12-03 Last updated: 2010-08-03Bibliographically approved

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Leygraf, ChristoferWallinder, Inger Odnevall

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