A microfluidic device for parallel 3-D cell cultures in asymmetric environments
2007 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 28, no 24, 4705-4712 p.Article in journal (Refereed) Published
We demonstrate a concept for how a miniaturized 3-D cell culture in biological extracellular matrix (ECM) or synthetic gels bridges the gap between organ-tissue culture and traditional 2-D cultures. A microfluidic device for 3-D cell culture including microgradient environments has been designed, fabricated, and successfully evaluated. In the presented system stable diffusion gradients can be generated by application of two parallel fluid flows with different composition against opposite sides of a gel plug with embedded cello. Culture for up to two weeks was performed showing cells still viable and proliferating. The cell tracer dye calcein was used to verify gradient formation as the fluorescence intensity in exposed cells was proportional to the position in the chamber. Cellular response to an applied stimulus was demonstrated by use of an adenosine triphosphate gradient where the onset of a stimulated intracellular calcium release also depended on cell position.
Place, publisher, year, edition, pages
2007. Vol. 28, no 24, 4705-4712 p.
3-D cell culture; extracellular matrix; gradient; hydrogel; microfluidics
IdentifiersURN: urn:nbn:se:kth:diva-11816DOI: 10.1002/elps.200700342ISI: 000252465600022PubMedID: 18070345ScopusID: 2-s2.0-37548999753OAI: oai:DiVA.org:kth-11816DiVA: diva2:283452
QC 201007232009-12-282009-12-282010-11-19Bibliographically approved