FR-like EBNA1 binding repeats in the human genome
2010 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 405, no 2, 524-529 p.Article in journal (Refereed) Published
Epstein Barr Virus (EBV) is widely spread in the human population. EBV nuclear antigen 1 (EBNA1) is a transcription factor that activates viral genes and is necessary for viral replication and partitioning, which binds the EBV genome cooperatively. We identify similar EBNA1 repeat binding sites in the human genome using a nearest-neighbour positional weight matrix. Previously experimentally verified EBNA1 sites in the human genome are successfully recovered by our approach. Most importantly, 40 novel regions are identified in the human genome, constituted of tandemly repeated binding sites for EBNA1. Genes located in vicinity of these regions are presented as possible targets for EBNA1-mediated regulation. Among these, four are discussed in more detail: IQCB1, IMPG1, IRF2BP and TPO. Incorporating the cooperative actions of EBNA1 is essential when identifying regulatory regions in the human genome and we believe the findings presented here are highly valuable for the understanding of EBV-induced phenotypic changes.
Place, publisher, year, edition, pages
2010. Vol. 405, no 2, 524-529 p.
EBNA1, Epstein-Barr virus, Family of Repeats, Human binding sites
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:kth:diva-12093DOI: 10.1016/j.virol.2010.06.040ISI: 000281130500028PubMedID: 20655080ScopusID: 2-s2.0-77955661979OAI: oai:DiVA.org:kth-12093DiVA: diva2:301264
QC 20100720. Updated from submitted to published 201203262010-03-032010-03-032012-03-26Bibliographically approved