The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications
2006 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 6, no 8, 1682-1686 p.Article in journal (Refereed) Published
We investigated the effect of spherical gold nanoparticles on immature dendritic cells (DCs). Conventionally produced nanoparticles had a maturating effect on the DCs-a result of lipopolysaccharide (LPS) contamination. By modification of the production process, low-LPS particles were obtained, which had practically no effect on phenotypic maturation or cytokine production of the DCs. Our findings emphasize the importance of high purity in the production of nanoparticles, since possible contaminants may interfere with the assessment of biological/medical effects. They also highlight that nanoparticles can function as carriers of immune modulating contaminants.
Place, publisher, year, edition, pages
2006. Vol. 6, no 8, 1682-1686 p.
human dendritic cells, gold, immunity, lipopolysaccharide, contamination, maturation, particles, receptor, innate, metal
IdentifiersURN: urn:nbn:se:kth:diva-12100DOI: 10.1021/nl060860zISI: 000239623900019ScopusID: 2-s2.0-33748329694OAI: oai:DiVA.org:kth-12100DiVA: diva2:301413
QC 201007212010-03-032010-03-032010-11-15Bibliographically approved