Electrostatic Interactions of Fluorescent Molecules with Dielectric Interfaces Studied by Total Internal Reflection Fluorescence Correlation Spectroscopy
2010 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, Vol. 11, no 2, 368-406 p.Article in journal (Refereed) Published
Electrostatic interactions between dielectric surfaces and different fluorophoresused in ultrasensitive fluorescence microscopy are investigated using objective-based TotalInternal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacialdynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein,zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengthsat physiological pH. As analyzed by means of the amplitude and time-evolution of theautocorrelation function, the fluorescent molecules experience electrostatic attraction orrepulsion at the glass surface depending on their charges. Influences of the electrostaticinteractions are also monitored through the triplet-state population and triplet relaxationtime, including the amount of detected fluorescence or the count-rate-per-moleculeparameter. These TIR-FCS results provide an increased understanding of how fluorophoresare influenced by the microenvironment of a glass surface, and show a promising approachfor characterizing electrostatic interactions at interfaces.
Place, publisher, year, edition, pages
MDPI , 2010. Vol. 11, no 2, 368-406 p.
electrostatic interactions, fluorescent molecules, glass interfaces, Total Internal Reflection, Fluorescence Correlation Spectroscopy
IdentifiersURN: urn:nbn:se:kth:diva-12340DOI: 10.3390/ijms11020386ISI: 000274973500001ScopusID: 2-s2.0-77649207561OAI: oai:DiVA.org:kth-12340DiVA: diva2:309801
FunderSwedish Research Council, VR-621-2006-3197Knut and Alice Wallenberg Foundation
QC 201006172010-04-122010-04-082011-01-20Bibliographically approved