Visual DNA as a diagnostic tool
2009 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 30, no 21, 3691-3695 p.Article in journal (Refereed) Published
We report on the incorporation of the Visual DNA concept in a genotyping assay as a simple and straightforward detection tool. The principle of trapping streptavidin-coated superparamagnetic beads of micrometer size for visualization of genetic variances is used for PrASE-based detection of a panel of mutations in the severe and common genetic disorder of cystic fibrosis. The method allows a final investigation of genotypes by the naked eye and the output is easily documented using a regular hand-held device with an integrated digital camera. A number of samples were run through the assay, showing rapid and accurate detection using superparamagnetic beads and an off-the-shelf neodymium magnet. The assay emphasizes the power of Visual DNA and demonstrates the potential value of the method in future point-of-care tests.
Place, publisher, year, edition, pages
2009. Vol. 30, no 21, 3691-3695 p.
Cystic fibrosis, Diagnostics, Mutation, PrAse, Visual DNA, CYSTIC-FIBROSIS GENE, IDENTIFICATION, TECHNOLOGIES
IdentifiersURN: urn:nbn:se:kth:diva-12401DOI: 10.1002/elps.200900273ISI: 000272126000006ScopusID: 2-s2.0-70649091306OAI: oai:DiVA.org:kth-12401DiVA: diva2:310836
qc 201004162010-04-162010-04-162010-06-18Bibliographically approved