Hydrodynamic Flow Profiling in Microchannel Structures by Single Molecule Fluorescence Correlation Spectroscopy
2000 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 72, no 14, 3260-3265 p.Article in journal (Refereed) Published
In this paper we demonstrate high spatial resolution hydrodynamic flow profiling in silicon wafer based microchannels using single molecule fluorescence correlation spectroscopy (FCS). We have used confocal fluorescence microscopy to detect single tetramethylrhodamine (TMR-4-dUTP) biomolecules traversing a l fL volume element defined by an argon laser beam focus. By elevating a (10-10 M) reservoir of diluted analyte, a continuous hydrodynamic flow through the microstructure could be accomplished. The microchannel was then scanned with a diffraction-limited focus in 1-μm steps in both the vertical and the horizontal directions to determine the flow profile across a 50 × 50 μm2 channel. The flow profile measured was parabolic in both dimensions, thereby showing a Poiseuille laminar flow profile. Future microstructures can hereby be nondestructively investigated with the use of high spatial resolution confocal correlation microscopy.
Place, publisher, year, edition, pages
2000. Vol. 72, no 14, 3260-3265 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-12417DOI: 10.1021/ac991448pISI: 000088347100038OAI: oai:DiVA.org:kth-12417DiVA: diva2:311144
QC 201004202010-04-202010-04-202011-02-09Bibliographically approved