Parallel Flow Measurements in Microstructures by Use of a Multifocal 4 x 1 Diffractive Optical Fan-Out Element
2002 (English)In: Applied Optics, ISSN 0003-6935, E-ISSN 1539-4522, Vol. 41, no 31, 6614-6620 p.Article in journal (Refereed) Published
We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 μm x 20 μm square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied.
Place, publisher, year, edition, pages
2002. Vol. 41, no 31, 6614-6620 p.
fluorescence correlation spectroscopy, cross-correlation, gene-expression, arrays, identification, excitation, diffusion, genomics, design, laser
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-12447DOI: 10.1364/AO.41.006614ISI: 000178847600010OAI: oai:DiVA.org:kth-12447DiVA: diva2:311763
QC 201004232010-04-232010-04-232011-02-08Bibliographically approved