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Parallel Flow Measurements in Microstructures by Use of a Multifocal 4 x 1 Diffractive Optical Fan-Out Element
KTH, Superseded Departments, Microelectronics and Information Technology, IMIT.ORCID iD: 0000-0002-5584-9170
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2002 (English)In: Applied Optics, ISSN 1559-128X, E-ISSN 2155-3165, Vol. 41, no 31, 6614-6620 p.Article in journal (Refereed) Published
Abstract [en]

We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 μm x 20 μm square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied.

Place, publisher, year, edition, pages
2002. Vol. 41, no 31, 6614-6620 p.
Keyword [en]
fluorescence correlation spectroscopy, cross-correlation, gene-expression, arrays, identification, excitation, diffusion, genomics, design, laser
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-12447DOI: 10.1364/AO.41.006614ISI: 000178847600010OAI: oai:DiVA.org:kth-12447DiVA: diva2:311763
Note
QC 20100423Available from: 2010-04-23 Created: 2010-04-23 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Correlation spectrosopy of single eitters: fundamental studies and applications related to quantum optics and life science
Open this publication in new window or tab >>Correlation spectrosopy of single eitters: fundamental studies and applications related to quantum optics and life science
2003 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Correlation analysis and correlation spectroscopy has eversince the first developments, to characterise light emittingprocesses and biomolecular dynamics, continued to extend itspractical applicability. Today, correlation spectroscopy can beused in life science to study dynamical processes even at thesingle molecule level. Correlation analysis can in one of itsextreme be applied to investigate single photon processes fromsolid-state emitters. This thesis is an account of my studiesof fluorescent emitter related to quantum optics and lifescience. It presents some fundamental results and discussesapplicationsof emitters like single quantum dots or singledyes attached to biomolecules. The studies were performed bythe means of correlation analysis and correlation spectroscopyon self-made optical setups. One task of this thesis was todevelop fluorescence correlation spectroscopy for ultravioletexcitation and emission. With ultraviolet excitation thenatural intrinsic chromophores of certain nucleotides and aminoacids can be used. No external labelling of biomolecules couldbecome a reality using ultraviolet excitation and emission. Asecond task was to apply correlation spectroscopy to performhigh spatial-resolution flow profiling and trafficking ofsingle dye-labeled biomolecules in microstructured channels.Future transports effects, flow monitoring, flow profiling andprolonged fluorescence detection in artificial microstructuresor in cells, could benefit from this application. An additionaltask was to apply correlation spectroscopy to so-calledmicroarrays for parallel acquisition of dynamical data at thesingle molecule level. Parallel excitation and detection wasachieved with the use of diffractive optical elements andintegrated semiconductor single-photon sensitive detectors. Thecurrent throughput rate in biological diagnostic or screeninganalysis could be increased dramatically with implementation ofthis parallel confocal excitation and detection technique. Yetanother task of this thesis was to investigations single-photongeneration by InAs-semiconductor quantum dots. We show that aquantum dots can be used for single-photon generation ondemand. Besides the single-photon generation in quantum dots,the possibility of two-photon generation, and generation ofentangled photon-pair, has also been investigated.

Place, publisher, year, edition, pages
Stockholm: KTH, 2003. xviii, 85 p.
Series
Trita-MVT, ISSN 0348-4467 ; 2003:7
Keyword
optics, analytical chemistry, solid states
National Category
Engineering and Technology
Identifiers
urn:nbn:se:kth:diva-3593 (URN)91-7283-520-6 (ISBN)
Public defence
2003-09-23, 00:00 (English)
Note
QC 20100423 NR 20140805Available from: 2003-09-16 Created: 2003-09-16 Last updated: 2010-04-28Bibliographically approved

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