Detection of mutations in PCR products from clinical samples by surface plasmon resonance
1997 (English)In: Journal of Molecular Recognition, ISSN 0952-3499, E-ISSN 1099-1352, Vol. 10, no 1, 7-17 p.Article in journal (Refereed) Published
Two different strategies for scanning and screening of mutations in polymerase chain reaction (PCR) products by hybridization analysis are described, employing real-time biospecific interaction analysis (BIA) for detection, Real-time BIA was used to detect differences in hybridization responses between PCR products and different 17-mer oligonucleotide probes, For the analysis using a biosensor instrument, two different experimental formats were investigated based on immobilization of either biotinylated PCR products or oligonucleotide probes onto a sensor chip, Applied on the human tumour suppressor p53 gene, differences in hybridization levels for full-match and mismatch situations employing both formals allowed the detection of point mutations in exon 6 PCR products, derived from a breast tumour biopsy sample, In addition, a mutant sample sequence could be detected in a 50/50 background of wild type exon 6 sequence, The suitability of the different formats for obtaining a regenerable system and a high throughput of samples is discussed.
Place, publisher, year, edition, pages
1997. Vol. 10, no 1, 7-17 p.
IdentifiersURN: urn:nbn:se:kth:diva-13320ISI: A1997XA36200002OAI: oai:DiVA.org:kth-13320DiVA: diva2:323686
QC 201006112010-06-112010-06-112010-06-11Bibliographically approved