REAL-TIME MONITORING OF DNA MANIPULATIONS USING BIOSENSOR TECHNOLOGY
1995 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 224, no 1, 400-408 p.Article in journal (Refereed) Published
The potential of real-time biospecific interaction analysis technology for applications in molecular biology is described. DNA fragments are immobilized onto a biosensor surface using the high-affinity streptavidin-biotin system and subsequently used to monitor different unit operations in molecular biology, e.g., DNA strand separation, DNA hybridization kinetics, and enzymatic modifications. A model system comprising six oligonucleotides was used, which can be assembled into a 69-bp double-stranded DNA fragment. Using this system, the biosensor approach was employed to analyze multistep solid-phase gene assembly and the performance of different enzymes routinely used for the synthesis and manipulation of DNA. In addition, a concept for the determination of single-point mutations in DNA samples is described.
Place, publisher, year, edition, pages
1995. Vol. 224, no 1, 400-408 p.
IdentifiersURN: urn:nbn:se:kth:diva-13317ISI: A1995QB30600057OAI: oai:DiVA.org:kth-13317DiVA: diva2:323703
QC 201006112010-06-112010-06-112014-09-24Bibliographically approved