Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Solid-phase method for differential display of genes expressed in hematopoietic stem cells.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0003-0996-1644
Show others and affiliations
1996 (English)In: BioTechniques, ISSN 0736-6205, E-ISSN 1940-9818, Vol. 21, no 1, 114-21 p.Article in journal (Refereed) Published
Abstract [en]

A solid-phase differential display method was designed to analyze differential gene expression in samples with low amounts of mRNA. The principle was based on using a biotinylated probe to capture the mRNA and priming both the first-strand synthesis and the subsequent polymerase chain reaction step. Coupling the mRNA to a solid phase during the procedure simplified the purification steps, limited sample loss and enabled rapid handling of mRNA. DNA contamination was also minimized when the mRNA was bound to a solid phase. Optimization of the differential display method was achieved by analyzing both the enzymatic conditions and the required cell amounts. The approach was used for the characterization of genes expressed in the most immature hematopoietic progenitor cells (CD34+CD38-). The majority of the differentially expressed fragments represented previously uncharacterized sequences.

Place, publisher, year, edition, pages
1996. Vol. 21, no 1, 114-21 p.
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-13361PubMedID: 8816245OAI: oai:DiVA.org:kth-13361DiVA: diva2:324515
Note
QC 20100615Available from: 2010-06-15 Created: 2010-06-15 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Analysis of human gene transcripts: Identification and characterisation based on homology and differential expression
Open this publication in new window or tab >>Analysis of human gene transcripts: Identification and characterisation based on homology and differential expression
1998 (English)Doctoral thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 1998. 68 p.
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-2751 (URN)91-7170-347-0 (ISBN)
Public defence
1998-12-18, 00:00
Note
QC 20100615Available from: 2000-01-01 Created: 2000-01-01 Last updated: 2010-06-17Bibliographically approved

Open Access in DiVA

No full text

PubMed

Search in DiVA

By author/editor
Odeberg, JacobLundeberg, Joakim
By organisation
Biotechnology
In the same journal
BioTechniques
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf