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Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biochemistry and Biotechnology.ORCID iD: 0000-0002-2993-9375
KTH, Superseded Departments, Biochemistry and Biotechnology.
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2002 (English)In: Bioprocess and biosystems engineering (Print), ISSN 1615-7591, E-ISSN 1615-7605, Vol. 24, no 6, 385-393 p.Article in journal (Refereed) Published
Abstract [en]

A fusion protein composed of a cellulose binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B (CBD-lipase) was produced by Pichia pastoris methanol utilization plus phenotype in high cell-density cultures. The genes expressing CBD-lipase were fused to the alpha-factor secretion signal sequence of Saccharomyces cerevisiae and placed under the control of the alcohol oxidase gene (AOX1) promoter. To control the repression and induction of AOX1 and oxygen demand at high cell density, a four-stage process was used. Batch growth on glycerol was used in the first step to provide biomass (28 g L-1) while product formation was prevented due to repression of the AOX1. The second stage was exponential fed-batch growth on glycerol, which caused a slight increase of the enzyme alcohol oxidase activity due to derepression of the AOX1. This procedure resulted in smooth transition to exponential fed-batch growth on methanol, the third stage, in which the AOX1 was strongly induced. The fourth stage was constant fed-batch growth on methanol used to control the oxygen demand at the high cell density. A kinetic model was developed that could predict biomass growth and oxygen consumption in processes with and without oxygen-enriched air. With oxygen enrichment to 34% O-2 in the inlet air the methanol feed rate could be increased by 50% and this resulted in 14% higher final cell density (from 140 to 160 g L-1 cell dry weight). The increased methanol feed rate resulted in a proportionally increased specific rate of product secretion to the medium. After an initial decrease, the synthesis capacity of the cell was kept constant throughout the cultivation, which made the product concentration increase almost constantly during the process. The kinetic model also describes how the low maintenance demand of P. pastoris compared with E. coli enables this organism to grow to such high cell densities.

Place, publisher, year, edition, pages
2002. Vol. 24, no 6, 385-393 p.
Keyword [en]
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-13453DOI: 10.1007/s00449-001-0274-5ISI: 000174810400006OAI: diva2:325393
QC 20100618Available from: 2010-06-18 Created: 2010-06-18 Last updated: 2012-06-07Bibliographically approved
In thesis
1. Process techniques for production of recombinant proteins with Picha pastoris
Open this publication in new window or tab >>Process techniques for production of recombinant proteins with Picha pastoris
2003 (English)Doctoral thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 2003. 51 p.
p. Pastoris, fed-batch culture, proteolysis, modeling
National Category
Engineering and Technology
urn:nbn:se:kth:diva-3572 (URN)91-7283-522-2 (ISBN)
Public defence
2003-06-13, 00:00
QC 20100618Available from: 2003-06-26 Created: 2003-06-26 Last updated: 2010-06-18Bibliographically approved

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