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Suitable materials for soft tissue reconstruction: In vitro studies of cell-triblock copolymer interactions
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2005 (English)In: Journal of bioactive and compatible polymers (Print), ISSN 0883-9115, E-ISSN 1530-8030, Vol. 20, no 6, 509-526 p.Article in journal (Refereed) Published
Abstract [en]

Keratinocytes and fibroblasts have been grown onto a series of triblock copolymers based on 1,5-dioxepan-2-one (DXO) and L-lactide (LLA). The molar ratio of DXO and LLA were varied in the copolymers. This resulted in different degrees of hydrophilicity, which in turn influenced the cell growth. On these surfaces, the morphological appearance of the cells with their cell movements and growth were investigated by means of scanning electron microscopy, time-lapse videomicroscopy and immunohistochemistry. All results clearly showed that the keratinocytes and fibroblasts adhered best to the most hydrophilic copolymers. A majority of the keratinocytes seeded on the most hydrophilic copolymer also presented a polarized morphology indicating a migration tendency. The cell growth onto these materials are interesting since a possible application for these unique materials is as polymeric membranes for guided cutaneous and/or periodontal tissue generation.

Place, publisher, year, edition, pages
2005. Vol. 20, no 6, 509-526 p.
Keyword [en]
triblock copolymers, hydrophilicity, cell culture, time-lapse video microscopy, cell morphology, cell movement, cell growth, tissue engineering, RING-OPENING POLYMERIZATION, L-LACTIDE, FUNCTIONAL-GROUPS, ADHESION, MIGRATION, BIOMATERIALS, 1, 5-DIOXEPAN-2-ONE, SURFACES, ORGANIZATION, BACTERIAL
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-13824DOI: 10.1177/0883911505058608ISI: 000232940700001ScopusID: 2-s2.0-27844591666OAI: diva2:327502
QC 20100629Available from: 2010-06-29 Created: 2010-06-29 Last updated: 2010-07-14Bibliographically approved
In thesis
1. Bioresorbable copolymers with tailored properies: innovative materials för soft tissuel engineering
Open this publication in new window or tab >>Bioresorbable copolymers with tailored properies: innovative materials för soft tissuel engineering
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The emerging need for new synthetic materials for soft tissue engineering applications encourages the search for innovative polymers having interesting properties. Ring-opening polymerization of lactones and lactides initiated by tin alkoxides has received particular attention due to the versatility of the method for building up well-defined biodegradable structures. The controlled reactions together with a careful choice of comonomers and copolymer composition make it possible to create materials with desired molecular architecture and properties.The aim of the work described in this thesis was to design aliphatic bioresorbable copolymers with new structures and controlled properties for potential application in soft tissue engineering. The first part of the work was focused on the surface properties of the materials synthesized for biomedical application. Solution-cast film triblock copolymers of L-lactide (LLA) and 1,5-dioxepan-2-one (DXO), subjected to thermal treatment have been studied. The effects of molecular weight, polymer composition, cooling rate, and casting solution concentration on the nanostructure surface morphology and topography have been investigated by atomic force microscopy (AFM). The surface characterization of the annealed triblock copolymers revealed well-defined fiber features formed as a result of a melt-induced micro-phase separation during crystallization. The dimensions and shape of the formations could be related to the copolymer composition and annealing conditions, and this makes it possible to create controlled and well-defined surface structure. The results of cell adhesion studies on annealed triblock copolymers indicate that these materials favor fibroblast growth and spreading, which makes them promising candidates for applications as bioresorbable membranes.In the next stage of the work, linear and network copolymers of ε-caprolactone (CL) and DXO with a controlled composition and controlled hydrophilicity have been synthesized. The molar fraction of DXO in the copolymers affected their mechanical, thermal and surface properties. The hydrophilicity was tailored by changing the monomer composition in the copolymers. The AFM measurements on the linear copolymers showed that short fibrillar structures were formed upon crystallization from the melt. The supple CL-DXO networks were easy to cast and could easily be removed from the mould surface, so that it is possible to use this material for embossing procedures without the risk of damaging the surface pattern during removal from the mould.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 70 p.
Trita-FPT-Report, ISSN 1652-2443 ; 2006:18
poly(1, 5-dioxepan-2-one), poly(ε-caprolactone), poly(L-lactide), poly(trimethylene carbonate), triblock, network, cyclic tin alkoxide, controlled coordination-insertion ring-opening polymerization, thermoplastic elastomers
National Category
Polymer Chemistry
urn:nbn:se:kth:diva-4042 (URN)91-7178-378-4 (ISBN)
Public defence
2006-06-16, Aal K2, Teknikringen 28, Stockholm, 10:00 (English)
QC 20100629Available from: 2006-06-07 Created: 2006-06-07 Last updated: 2010-06-29Bibliographically approved

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Albertsson, Ann-ChristineFinne, AnnaAndronova, Natalia
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