Suitable materials for soft tissue reconstruction: In vitro studies of cell-triblock copolymer interactions
2005 (English)In: Journal of bioactive and compatible polymers (Print), ISSN 0883-9115, E-ISSN 1530-8030, Vol. 20, no 6, 509-526 p.Article in journal (Refereed) Published
Keratinocytes and fibroblasts have been grown onto a series of triblock copolymers based on 1,5-dioxepan-2-one (DXO) and L-lactide (LLA). The molar ratio of DXO and LLA were varied in the copolymers. This resulted in different degrees of hydrophilicity, which in turn influenced the cell growth. On these surfaces, the morphological appearance of the cells with their cell movements and growth were investigated by means of scanning electron microscopy, time-lapse videomicroscopy and immunohistochemistry. All results clearly showed that the keratinocytes and fibroblasts adhered best to the most hydrophilic copolymers. A majority of the keratinocytes seeded on the most hydrophilic copolymer also presented a polarized morphology indicating a migration tendency. The cell growth onto these materials are interesting since a possible application for these unique materials is as polymeric membranes for guided cutaneous and/or periodontal tissue generation.
Place, publisher, year, edition, pages
2005. Vol. 20, no 6, 509-526 p.
triblock copolymers, hydrophilicity, cell culture, time-lapse video microscopy, cell morphology, cell movement, cell growth, tissue engineering, RING-OPENING POLYMERIZATION, L-LACTIDE, FUNCTIONAL-GROUPS, ADHESION, MIGRATION, BIOMATERIALS, 1, 5-DIOXEPAN-2-ONE, SURFACES, ORGANIZATION, BACTERIAL
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-13824DOI: 10.1177/0883911505058608ISI: 000232940700001ScopusID: 2-s2.0-27844591666OAI: oai:DiVA.org:kth-13824DiVA: diva2:327502
QC 201006292010-06-292010-06-292010-07-14Bibliographically approved