Sequence analysis of domestic dog mitochondrial DNA for forensic use
1997 (English)In: Journal of Forensic Sciences, ISSN 0022-1198, E-ISSN 1556-4029, Vol. 42, no 4, 593-600 p.Article in journal (Refereed) Published
A method has been developed for the direct sequencing of hypervariable region 1 (HV1) of domestic dog (Canis familiaris) and wolf (Canis lupus) mitochondrial DNA (mtDNA) using single hairs as template. The method uses a robotic work-station and an automated sequencer to allow for robust routine analysis. A population data base was created in order to investigate the forensic and population-genetic informativeness of domestic dog HV1. Sequence variation, partitioning of dog breeds among sequence variants and phylogenetic relations between the variants were determined. Samples from 102 domestic dogs of 52 different breeds and two captive wolves were analyzed. Nineteen dog sequence variants were found and the frequencies of the variants ranged from 1 to 21%. The calculated discrimination power of the region, i.e., the exclusion capacity, implied that nine out of ten disputed individuals can be excluded by this analysis. The sequence variants were found to cluster into four phylogenetic groups.
Place, publisher, year, edition, pages
1997. Vol. 42, no 4, 593-600 p.
forensic science, DNA typing, dog, Canis familiaris, mitochondrial DNA, sequence analysis, hair, SINGLE HAIRS, IDENTIFICATION, VARIABILITY, POPULATION, FAMILY, PCR
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:kth:diva-13928ISI: A1997XL34800004OAI: oai:DiVA.org:kth-13928DiVA: diva2:328263
QC 201007022010-07-022010-07-022010-07-02Bibliographically approved