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Sequence analysis of domestic dog mitochondrial DNA for forensic use
KTH, Superseded Departments, Biochemistry and Biotechnology.ORCID iD: 0000-0002-1495-8338
KTH, Superseded Departments, Biochemistry and Biotechnology.ORCID iD: 0000-0003-2745-6289
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1997 (English)In: Journal of Forensic Sciences, ISSN 0022-1198, E-ISSN 1556-4029, Vol. 42, no 4, 593-600 p.Article in journal (Refereed) Published
Abstract [en]

A method has been developed for the direct sequencing of hypervariable region 1 (HV1) of domestic dog (Canis familiaris) and wolf (Canis lupus) mitochondrial DNA (mtDNA) using single hairs as template. The method uses a robotic work-station and an automated sequencer to allow for robust routine analysis. A population data base was created in order to investigate the forensic and population-genetic informativeness of domestic dog HV1. Sequence variation, partitioning of dog breeds among sequence variants and phylogenetic relations between the variants were determined. Samples from 102 domestic dogs of 52 different breeds and two captive wolves were analyzed. Nineteen dog sequence variants were found and the frequencies of the variants ranged from 1 to 21%. The calculated discrimination power of the region, i.e., the exclusion capacity, implied that nine out of ten disputed individuals can be excluded by this analysis. The sequence variants were found to cluster into four phylogenetic groups.

Place, publisher, year, edition, pages
1997. Vol. 42, no 4, 593-600 p.
Keyword [en]
forensic science, DNA typing, dog, Canis familiaris, mitochondrial DNA, sequence analysis, hair, SINGLE HAIRS, IDENTIFICATION, VARIABILITY, POPULATION, FAMILY, PCR
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:kth:diva-13928ISI: A1997XL34800004OAI: oai:DiVA.org:kth-13928DiVA: diva2:328263
Note
QC 20100702Available from: 2010-07-02 Created: 2010-07-02 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Mitochondrial DNA: analysis of the control region in forensic and population genetic studies
Open this publication in new window or tab >>Mitochondrial DNA: analysis of the control region in forensic and population genetic studies
1999 (English)Doctoral thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 1999. 49 p.
Keyword
mitochondrial DNA, control region, tandem repeats, D-loop, homoplasmy, hair, forensic, microsatellites, evolution, DNA sequencing, PCR, domestic dog, wolf
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-2824 (URN)91-7170-408-6 (ISBN)
Public defence
1999-06-11, 00:00
Note
QC 20100702Available from: 2000-01-01 Created: 2000-01-01 Last updated: 2010-07-02Bibliographically approved

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Savolainen, PeterHolmberg, AndersUhlen, Mathias

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