Understanding promiscuous amidase activity of an esterase from Bacillus subtilis
2008 (English)In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 9, no 1, 67-69 p.Article in journal (Refereed) Published
Water works. Bacillus subtilis esterase BS2 is a promiscuous esterase that shows amidase activity. This amidase activity was shown to depend on a hydrogen-bond network with the substrate amide hydrogen (indicated by arrow). When this stabilising hydrogen bond network was removed by a point mutation, the amide activity was significantly lowered in comparison with the esterase activity. (Figure Presented)
Place, publisher, year, edition, pages
2008. Vol. 9, no 1, 67-69 p.
amidases, Bacillus subtilis, catalysis, esterases, molecular modeling
IdentifiersURN: urn:nbn:se:kth:diva-14181DOI: 10.1002/cbic.200700521ISI: 000252292200013OAI: oai:DiVA.org:kth-14181DiVA: diva2:331473
QC 201007222010-07-222010-07-222010-07-22Bibliographically approved