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An integrated QCM-based narcotics sensing microsystem
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).ORCID iD: 0000-0001-6443-878X
Biosensor Applications AB, Solna.
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).ORCID iD: 0000-0001-8248-6670
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2008 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 8, no 10, 1648-1657 p.Article in journal (Refereed) Published
Abstract [en]

We present the design, fabrication and successful testing of a 14 x 14 x 4 mm(3) integrated electronic narcotics sensing system which consists of only four parts. The microsystem absorbs airborne narcotics molecules and performs a liquid assay using an integrated quartz crystal microbalance (QCM). A vertically conductive double-sided adhesive foil (VCAF) was used and studied as a novel material for LOC and MEMS applications and provides easy assembly, electrical contacting and liquid containment. The system was tested for measuring cocaine and ecstasy, with successful detection of amounts as small as 100 ng and 200 ng, respectively These levels are of interest in security activities in customs, prisons and by the police.

Place, publisher, year, edition, pages
RSC Publishing, 2008. Vol. 8, no 10, 1648-1657 p.
Keyword [en]
electronic nose, quartz crystal microbalance, microsystem, narcotics detection, air-liquid interfacing, diaphragm, microfluidics, micro electro mechanical systems, molecular transport
National Category
Control Engineering
URN: urn:nbn:se:kth:diva-14189DOI: 10.1039/b800487kISI: 000260466300007ScopusID: 2-s2.0-52749089053OAI: diva2:331620

QC 20100723. Tidigare titel: An integrated narcotics sensing microsystem

Available from: 2010-07-23 Created: 2010-07-23 Last updated: 2015-06-18Bibliographically approved
In thesis
1. MEMS Interfaces for Bioanalysis Systems
Open this publication in new window or tab >>MEMS Interfaces for Bioanalysis Systems
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

This thesis deals with various aspects of using open microfluidic interfaces. Three specific areas of application are studied.

The first is air-to-liquid interfacing in biosensors with possibilities for component inte­gration. A micromachined interface for airborne sample-to-liquid and droplet-to-liquid adsorption is discussed. It enables a robust sheet liquid flow serving as adsorption site. The inter­face properties are presented. Along with the interface, a novel method and system for rapid detection of dust and vapour-based narcotics and explosives traces is introduced. The QCM sensor detection principle with antibody immunoassay is described. Having shown the working principles of molecular adsorption to liquid surface and molecular detection with QCM technology, an integrated device is introduced. Diffusion as an effective transport mechanism in this microfluidic device is discussed. By holding the two components (inter­face and QCM) together with a double-sided adhesive, anisotropically vertically conductive tape, we achieve three functions, namely fixation, electrical connection and liquid sealing. Finally, enhanced electrostatic trapping of small particles to the liquid interface is demon­strated.

The second area concerns open microfluidics for the integration of capillary electropho­resis and mass spectroscopy. A technique for hyphenation between CE and MALDI-MS is presented. Two closed fused-silica capillaries were connected via a silicon chip comprising an open microcanal. The influence of the capillary-to-microcanal connection is discussed, as well as a simple technique to control evaporation from the open microcanal.

The third area concerns microfluidics enabling studies of single cells in asymmetric en­vironments. Using extracellular matrix or synthetic gel-embedding cells in an assay chamber, cells thrive and proliferate. This makes it possible to carry out medium to long term cultiva­tion of cells in a more physiological, controlled 3D environment than in traditional 2D cul­tures. The gels are discussed in terms of handling as well as their properties. A gel and micro­fluidic device for three dimensional cell culture with microgradient environments is pre­sented. Finally, a method for studying cilia-forming cells in asymmetric microfluidic environments is presented. Bending the primary cilium with a fluid flow will give rise to a response, but sensitivity to flow direction has only been sparsely studied. Design considerations are presented and discussed.

Abstract [sv]

Den här avhandlingen diskuterar olika aspekter av den öppna gränsytan hos styckevis öppna mikrofluidiksystem. Tre specifika användningsområden har studerats.

Det första är gränsytan mellan luft och vätska i en biosensor och de användningsområ­den som finns här. Ett mikrofabricerat interface för adsorption av luftburna substanser samt dropp-absorption diskuteras. Här används en rörlig vätskeyta som adsorbtionsyta och dess egenskaper presenteras. En ny metod för sprängämnes- och narkotikadetektering med interfa­cet introduceras. QCM-tekniken i kombination med antikroppskemi beskrivs. En integrerad lösning med dessa tekniker introduceras där diffusion utgör en effektiv transportmekanism. Med en dubbelsidig ledande tejp hålls komponenterna ihop, tätas och förses med ström. Slut­ligen presenteras elektrostatisk infångning av partiklar där den ena elektroden utgörs av väts­keytan.

Det andra området berör ett öppet mikrofluidiksystem för integrering av kapillärelek­trofores och masspektrometri. Teknik för att koppla ihop CE och MALDI-MS presente­ras. Två glaskapillärer har kopplats ihop med ett kiselchip med en öppen mikrokanal. Kopp­lingen mellan kapillären och chippet diskuteras liksom en enkel teknik för att kontrol­lera avdunstningen från den öppna mikrokanalen.

Det tredje området diskuterar hur mikrofluidik möjliggör studier av cellulära reaktioner i asymmetriska miljöer. Med inbäddning av celler i extracellulär matris eller syntetisk gel fås fysiologiskt relevant lokal miljö för celltillväxt och celldelning. Detta möjliggör studier av cellutveckling och cellreaktioner under lång tid i faktisk 3D-miljö till skillnad från den nuva­rande etablerade 2D-miljön. Gelerna diskuteras ur en hanteringssynpunkt liksom utifrån sina egenskaper. Ett system för cellodling i 3D med gradi­entmiljö presenteras och diskuteras. Slutligen presenteras ett system för studier av ciliefor­mande cellers respons där asymmetriska flöden ger upphov till böjning av cilier. Olika de­signaspekter diskuteras.

Place, publisher, year, edition, pages
Stockholm: KTH, 2008. xii, 56 p.
Trita-EE, ISSN 1653-5146 ; 2008:002
microfluidic interfacing, microfluidics, µTAS, sample transfer, biosensor, electronic nose, surface tension, quartz crystal microbalance, QCM, narcotics detection
National Category
Control Engineering
urn:nbn:se:kth:diva-4609 (URN)978-91-7178-846-7 (ISBN)
Public defence
2008-02-08, Sal M3, KTH, Brinellvägen 64, Stockholm, 10:00
QC 20100927Available from: 2008-01-18 Created: 2008-01-18 Last updated: 2010-09-27Bibliographically approved
2. Integrating Biosensors for Air Monitoring and Breath-Based Diagnostics
Open this publication in new window or tab >>Integrating Biosensors for Air Monitoring and Breath-Based Diagnostics
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The air we breathe is the concern of all of us but nevertheless we only know very little about airborne particles, and especially which biological microorganisms they contain. Today, we live in densely populated societies with a growing number of people, making us particularly vulnerable to air transmission of pathogens. With the recent appearance of highly pathogenic types of avian influenza in southeast Asia and the seasonal outbreaks of gastroenteritis caused by the extremely contagious norovirus, the need for portable, sensitive and rapid instruments for on-site detection and monitoring of airborne pathogens is apparent.

Unfortunately, the integration incompatibility between state-of-the-art air sampling techniques and laboratory based analysis methods makes instruments for in-the-field rapid detection of airborne particles an unresolved challenge.

This thesis aims at addressing this challenge by the development of novel manufacturing, integration and sampling techniques to enable the use of label-free biosensors for rapid and sensitive analysis of airborne particles at the point-of-care or in the field.

The first part of the thesis introduces a novel reaction injection molding technique for the fabrication of high quality microfluidic cartridges. In addition, electrically controlled liquid aspiration and dispensing is presented, based on the use of a thermally actuated polymer composite integrated with microfluidic cartridges.

The second part of the thesis demonstrates three different approaches of biosensor integration with microfluidic cartridges, with a focus on simplifying the design and integration to enable disposable use of the cartridges.

The third part to the thesis presents a novel air sampling technique based on electrophoretic transport of airborne particles directly to microfluidic cartridges. This technique is enabled by the development of a novel microstructured component for integrated air-liquid interfacing. In addition, a method for liquid sample mixing with magnetic microbeads prior to downstream biosensing is demonstrated.In the fourth part of the thesis, three different applications for airborne particle biosensing are introduced and preliminary experimental results are presented.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2015. xix, 85 p.
TRITA-EE, ISSN 1653-5146 ; 2015:020
National Category
Medical Laboratory and Measurements Technologies
urn:nbn:se:kth:diva-165454 (URN)978-91-7595-560-5 (ISBN)
Public defence
2015-05-22, Q2, Osquldas väg 10, KTH, Stockholm, 10:00 (English)
EU, FP7, Seventh Framework ProgrammeVINNOVASwedish Research CouncilSwedish Foundation for Strategic Research

QC 20150429

Available from: 2015-04-29 Created: 2015-04-28 Last updated: 2015-04-29Bibliographically approved

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