Antibody suspension bead arrays within serum proteomics
2008 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 7, no 8, 3168-3179 p.Article in journal (Refereed) Published
Antibody microarrays offer a powerful tool to screen for target proteins in complex samples. Here, we describe an approach for systematic analysis of serum, based on antibodies and using color-coded beads for the creation of antibody arrays in suspension. This method, adapted from planar antibody arrays, offers a fast, flexible, and multiplexed procedure to screen larger numbers of serum samples, and no purification steps are required to remove excess labeling substance. The assay system detected proteins down to lower picomolar levels with dynamic ranges over 3 orders of magnitude. The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins.
Place, publisher, year, edition, pages
2008. Vol. 7, no 8, 3168-3179 p.
antibody microarray, labeling, serum analysis, suspension bead arrays, antibody proteomics
IdentifiersURN: urn:nbn:se:kth:diva-14255DOI: 10.1021/pr700890bISI: 000258200400011ScopusID: 2-s2.0-53049097707OAI: oai:DiVA.org:kth-14255DiVA: diva2:331930
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201007282010-07-282010-07-282011-12-06Bibliographically approved