Change search
ReferencesLink to record
Permanent link

Direct link
The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures
Show others and affiliations
2005 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 26, no 3, 501-510 p.Article in journal (Refereed) Published
Abstract [en]

The biotin-streptavidin system is the strongest noncovalent biological interaction known, having a dissociation constant, K-d, in the order of 4 x 10(-14) m. The strength and specificity of the interaction has led it to be one of the most widely used affinity pairs in molecular, immunological, and cellular assays. However, it has previously been impossible to re-use any streptavidin solid support, since the conditions needed to break the interaction with biotin has led to the denaturation of the streptavidin. Here, we show that a short incubation in nonionic aqueous solutions at temperatures above 70degreesC can efficiently break the interaction without denaturing the streptavidin tetramer. Both biotin and the streptavidin remain active after dissociation and both molecules can therefore be re-used. The efficiency of the regeneration allowed solid supports with streptavidin to be used many times, here exemplified with the multiple re-use of streptavidin beads used for sample preparation prior to automated DNA sequencing. The results suggest that streptavidin regeneration can be introduced as an improvement in existing methods and assays based on the streptavidin system as well as emerging solid phase applications in fields, such as microfluiclics and nanotechnology.

Place, publisher, year, edition, pages
2005. Vol. 26, no 3, 501-510 p.
Keyword [en]
biotin, dissociation, magnetic beads, streptavidin, coded affinity tags, structural consequences, binding, avidin, purification, complex, dissociation, proteins, release, mutagenesis
URN: urn:nbn:se:kth:diva-14546DOI: 10.1002/elps.200410070ISI: 000227137300001ScopusID: 2-s2.0-14944364973OAI: diva2:332587
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Lundeberg, JoakimUhlén, Mathias
By organisation
Gene TechnologyProteomics
In the same journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 60 hits
ReferencesLink to record
Permanent link

Direct link