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Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides
KTH, School of Biotechnology (BIO), Glycoscience.
KTH, School of Biotechnology (BIO), Glycoscience.
2005 (English)In: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 15, no 4, 437-445 p.Article in journal (Refereed) Published
Abstract [en]

A novel class of nonionic, carbohydrate-based surfactants has been synthesized from the plant polysaccharide xyloglucan. Enzymatic hydrolysis of xyloglucan yielded a series of well-defined, highly branched oligosaccharides that, following reductive amination, were readily conjugated with fatty acids bearing C-8 to C-18 chains under mild conditions. The critical micelle concentration, determined by tensiometry and dye-inclusion measurements, showed a typical dependence on acyl chain length and was sensitive to the degree of galactosylation of the head group. Several compounds from this new group of surfactants, especially those with C-14 and C-16 chains, were useful for the extraction of membrane-bound enzyme markers from different plant cell compartments in catalytically active form.

Place, publisher, year, edition, pages
2005. Vol. 15, no 4, 437-445 p.
Keyword [en]
membrane protein extraction, reductive amination, surfactant, xyloglucan, xyloglucan oligosaccharides, sugar-based surfactants, beta-glucan synthesis, plant-cell walls, in-vitro, structural-analysis, callose synthase, interfacial behavior, product entrapment, plasma-membrane, cotton fiber
URN: urn:nbn:se:kth:diva-14611DOI: 10.1093/glycob/cwi013ISI: 000227724400010ScopusID: 2-s2.0-22444442865OAI: diva2:332652
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

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