Change search
ReferencesLink to record
Permanent link

Direct link
General strategies for efficient adjuvant incorporation of recombinant subunit immunogens
KTH, School of Biotechnology (BIO).
KTH, School of Biotechnology (BIO), Molecular Biotechnology.
KTH, School of Biotechnology (BIO).
Show others and affiliations
2005 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 23, no 17-18, 2331-2335 p.Article in journal (Refereed) Published
Abstract [en]

We have previously reported strategies for Escherichia coli production of recombinant immunogens fused to hydrophobic peptides or lipid tags to improve their capacity to be incorporated into an adjuvant formulation, e.g., immunostimulating complexes (iscoms). Recently, we also explored the strong interaction between biotin and streptavidin to achieve iscom association of recombinant immunogens. Plasmodium falciparum, Toxoplasma gondii and Neospora caninum antigens have served as model immunogens in the different studies. Generated fusion proteins have been found to be successfully incorporated into iscoms and high-titer antigen-specific antibody responses have been obtained upon immunization of mice. We believe that the different concepts presented, utilizing either hydrophobic peptide or lipid tags, or the recently explored biotin-streptavidin principle, offer convenient methods to achieve efficient adjuvant incorporation of recombinant immunogens.

Place, publisher, year, edition, pages
2005. Vol. 23, no 17-18, 2331-2335 p.
Keyword [en]
adjuvant, affinity purification, iscom, subunit vaccine, direct iscom incorporation, protein, systems
National Category
Immunology Medical Biotechnology
URN: urn:nbn:se:kth:diva-14630DOI: 10.1016/j.vaccine.2005.01.035ISI: 000227898700060ScopusID: 2-s2.0-14844303871OAI: diva2:332671
QC 20100525 QC 20111012. 4th World Congress on Vaccines and Immunisation (WCVI). Tokyo, JAPAN. SEP 30-OCT 03, 2004 Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2011-10-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Wikman, MariaFriedman, MikaelaAndersson, ChristinStåhl, Stefan
By organisation
School of Biotechnology (BIO)Molecular Biotechnology
In the same journal
ImmunologyMedical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 30 hits
ReferencesLink to record
Permanent link

Direct link