General strategies for efficient adjuvant incorporation of recombinant subunit immunogens
2005 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 23, no 17-18, 2331-2335 p.Article in journal (Refereed) Published
We have previously reported strategies for Escherichia coli production of recombinant immunogens fused to hydrophobic peptides or lipid tags to improve their capacity to be incorporated into an adjuvant formulation, e.g., immunostimulating complexes (iscoms). Recently, we also explored the strong interaction between biotin and streptavidin to achieve iscom association of recombinant immunogens. Plasmodium falciparum, Toxoplasma gondii and Neospora caninum antigens have served as model immunogens in the different studies. Generated fusion proteins have been found to be successfully incorporated into iscoms and high-titer antigen-specific antibody responses have been obtained upon immunization of mice. We believe that the different concepts presented, utilizing either hydrophobic peptide or lipid tags, or the recently explored biotin-streptavidin principle, offer convenient methods to achieve efficient adjuvant incorporation of recombinant immunogens.
Place, publisher, year, edition, pages
2005. Vol. 23, no 17-18, 2331-2335 p.
adjuvant, affinity purification, iscom, subunit vaccine, direct iscom incorporation, protein, systems
Immunology Medical Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-14630DOI: 10.1016/j.vaccine.2005.01.035ISI: 000227898700060ScopusID: 2-s2.0-14844303871OAI: oai:DiVA.org:kth-14630DiVA: diva2:332671
QC 20100525 QC 20111012. 4th World Congress on Vaccines and Immunisation (WCVI). Tokyo, JAPAN. SEP 30-OCT 03, 2004 2010-08-052010-08-052011-10-12Bibliographically approved