Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography
2005 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 19, no 7, 947-954 p.Article in journal (Refereed) Published
Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated.
Place, publisher, year, edition, pages
2005. Vol. 19, no 7, 947-954 p.
flight mass-spectrometry, gel-permeation chromatography, synthetic-polymers, substituent distribution, o-methylcelluloses, light-scattering, methyl cellulose, rapid method, ethers, combination
IdentifiersURN: urn:nbn:se:kth:diva-14633DOI: 10.1002/rcm.1874ISI: 000227907600012OAI: oai:DiVA.org:kth-14633DiVA: diva2:332674
QC 201005252010-08-052010-08-052011-07-13Bibliographically approved