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Antibody-based proteomics for human tissue profiling
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-8993-048X
2005 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 4, no 4, 384-393 p.Article, review/survey (Refereed) Published
Abstract [en]

Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture (pull-down) reagents for purification of specific proteins and their associated complexes for structural and biochemical analyses. In this review, the use of antibodies for such analysis will be discussed with focus on the possibility to create a descriptive and comprehensive protein atlas for tissue distribution and subcellular localization of human proteins in both normal and disease tissues.

Place, publisher, year, edition, pages
2005. Vol. 4, no 4, 384-393 p.
Keyword [en]
monoclonal-antibodies, functional genomics, escherichia-coli, breast-cancer, protein, microarray, expression, gene, immunohistochemistry, diagnostics
Identifiers
URN: urn:nbn:se:kth:diva-14639DOI: 10.1074/mcp.R500009-MCP200ISI: 000227980600005Scopus ID: 2-s2.0-17844396912OAI: oai:DiVA.org:kth-14639DiVA: diva2:332680
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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Uhlén, Mathias

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