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Fluorescence fluctuation spectroscopy in subdiffraction focal volumes
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.ORCID iD: 0000-0002-5584-9170
2005 (English)In: Physical Review Letters, ISSN 0031-9007, Vol. 94, no 17Article in journal (Refereed) Published
Abstract [en]

We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence intensity distribution analysis to extract molecular information about mobilities and fluorescence emission in solution. The combination of correlation analysis with that of photon intensity distributions reveals a fivefold squeezing of the detection volume over current diffraction-limited systems, which is in full agreement with the simultaneously demonstrated 25-fold reduction in (axial) focal transit time. Our method significantly extends the potential of far-field FFS, including for the noninvasive investigation of molecular reactions at higher concentrations.

Place, publisher, year, edition, pages
2005. Vol. 94, no 17
Keyword [en]
internal-reflection fluorescence, stimulated-emission, microscopy, breaking, barrier
URN: urn:nbn:se:kth:diva-14732DOI: 10.1103/PhysRevLett.94.178104ISI: 000228932200072OAI: diva2:332773
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

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Blom, Hans
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