Change search
ReferencesLink to record
Permanent link

Direct link
Insertion sequence profiling of UK Mycoplasma bovis field isolates
KTH, School of Biotechnology (BIO), Proteomics.
Show others and affiliations
2005 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 107, no 04-mar, 301-306 p.Article in journal (Refereed) Published
Abstract [en]

The presence and distribution of insertion sequences ISMbov2 and ISMbov3 within Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221 bp and 185 bp, to detect ISMbov2 and ISMbov3, respectively, amplified from the homologous sequences ISMmyl and ISI634 within Mycoplasma mycoides subspecies mycoides small colony type. We present data obtained from 49 field isolates of M. bovis, originating from pneumonic lungs, collected within the United Kingdom between 1996 and 2002. Hybridisation profiles show considerable variation between strains. ISMbov2 sequences are present between 2 and 17 copies while there are between 3 and 14 copies of the ISI634 homologue ISMbov3. These data also provide support for previous analysis by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Crown

Place, publisher, year, edition, pages
2005. Vol. 107, no 04-mar, 301-306 p.
Keyword [en]
insertion sequence (IS), southern blotting, Mycoplasma bovis, genome, agent, dna
URN: urn:nbn:se:kth:diva-14754DOI: 10.1016/j.vetmic.2005.02.003ISI: 000229161400017ScopusID: 2-s2.0-18044363833OAI: diva2:332795
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Persson, Anja
By organisation
In the same journal
Veterinary Microbiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 22 hits
ReferencesLink to record
Permanent link

Direct link