Affinity ligands for industrial protein purification
2005 (English)In: Protein peptide letters, ISSN 0929-8665, Vol. 12, no 4, 305-310 p.Article, review/survey (Refereed) Published
Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
Place, publisher, year, edition, pages
2005. Vol. 12, no 4, 305-310 p.
Affinity chromatography, cleaning-in-place (CIP), deamidation, protein A, protein G, protein engineering, stabilization, escherichia-coli, synthetic ligand, deamidation, chromatography, peptides, residues, stability, design, immobilization, mutagenesis
IdentifiersURN: urn:nbn:se:kth:diva-14755ISI: 000229162900001ScopusID: 2-s2.0-19644367840OAI: oai:DiVA.org:kth-14755DiVA: diva2:332796
QC 201005252010-08-052010-08-05Bibliographically approved