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Enzymatic protein digest in chip-based nanovials with immobilized proteolytic enzymes
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry.ORCID iD: 0000-0002-3444-9987
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Analytical Chemistry.
2005 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 542, no 2, 137-143 p.Article in journal (Refereed) Published
Abstract [en]

In the present work, protein digest reactions in silicon-based microchips, coated with immobilized proteolytic enzymes, have been carried out. The performance of such vials, modified with trypsin or chymotrypsin, was tested with myoglobin as a substrate. Capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry were utilized for analysis of the digests, and the influence of different instrumentation setups. immobilization procedures and reaction conditions are discussed.

Place, publisher, year, edition, pages
2005. Vol. 542, no 2, 137-143 p.
Keyword [en]
protein digest, immobilized enzymes, nanovials, capillary electrophoresis, MALDI-TOF MS, capillary-electrophoresis, mass-spectrometry, complex, trypsin, identification
URN: urn:nbn:se:kth:diva-14841DOI: 10.1016/j.aca.2005.03.062ISI: 000229889400001ScopusID: 2-s2.0-20444390515OAI: diva2:332882
QC 20100525Available from: 2010-08-05 Created: 2010-08-05Bibliographically approved

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