Improved chemical analysis of cellulose ethers using dialkylamine derivatization and mass spectrometry
2005 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 6, no 5, 2793-2799 p.Article in journal (Refereed) Published
Oligosaccharides of hydroxypropylmethyl cellulose, hydroxypropyl cellulose, and methyl cellulose were investigated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The cellulose ether oligosaccharides were produced either by enzymatic depolymerization utilizing the purified family 5 endoglucanase from Bacillus agaradhaerens or by partial acidic depolymerization. To lower the limit of detection in MALDI-MS three dilakylamines, dimethyl-, diethyl-, and dipropylamine were studied as reagents for reductive amination of the oligosaccharides. All three amines contributed to a significant increase in sensitivity in MALDI-MS, especially for oligosaccharides with a degree of polymerization (DP) < 3. These reagents were also attractive due to their high volatility, which facilitated the purification of the reaction mixtures. It was established that low-mass discrimination in MALDI-MS in the DP range 1-7 was substantially reduced with dialkylamine derivatization. Hence, dialkylamine derivatization of cellulose ether oligosaccharides obtained by endoglucanase depolymerization increased the number of detected analyte components. Dimethylamine was concluded to be the preferred reagent of those evaluated.
Place, publisher, year, edition, pages
2005. Vol. 6, no 5, 2793-2799 p.
assisted-laser-desorption/ionization, acid 2-(diethylamino)ethyl ester, high-sensitivity detection, electrospray-ionization, sample preparation, o-methylcelluloses, methyl cellulose, oligosaccharides, thermogelation, chromatography
IdentifiersURN: urn:nbn:se:kth:diva-15044DOI: 10.1021/bm050270fISI: 000231899200051OAI: oai:DiVA.org:kth-15044DiVA: diva2:333085
QC 201005252010-08-052010-08-05Bibliographically approved