Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes
KTH, School of Biotechnology (BIO).
KTH, School of Biotechnology (BIO).
KTH, School of Biotechnology (BIO).
Show others and affiliations
2005 (English)In: Bioprocess and biosystems engineering (Print), ISSN 1615-7591, E-ISSN 1615-7605, Vol. 27, no 6, 399-406 p.Article in journal (Refereed) Published
Abstract [en]

An oxygen-limited fed-batch technique (OLFB) was compared to traditional methanol-limited fed-batch technique (MLFB) for the production of recombinant Thai Rosewood beta-glucosidase with Pichia pastoris. The degree of energy limitation, expressed as the relative rate of respiration (q(O)/q(O,max)), was kept similar in both the types of processes. Due to the higher driving force for oxygen transfer in the OLFB, the oxygen and methanol consumption rates were about 40% higher in the OLFB. The obligate aerobe P. pastoris responded to the severe oxygen limitation mainly by increased maintenance demand, measured as increased carbon dioxide production per methanol, but still somewhat higher cell density (5%) and higher product concentrations (16%) were obtained. The viability was similar, about 90-95%, in both process types, but the amount of total proteins released in the medium was much less in the OLFB processes resulting in substantially higher (64%) specific enzyme purity for input to the downstream processing.

Place, publisher, year, edition, pages
2005. Vol. 27, no 6, 399-406 p.
Keyword [en]
oxygen-limited fed batch (OLFB), methanol-limited fed batch (MLFB), Pichia pastoris, b-glucosidase, methanol, expression, yeast, growth, yield, oxidation
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-15170DOI: 10.1007/s00449-005-0005-4ISI: 000233136000006Scopus ID: 2-s2.0-28244476895OAI: oai:DiVA.org:kth-15170DiVA: diva2:333211
Note

QC 20100525

Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Process design for production of Thai rosewood β-glucosidase in Pichia pastoris
Open this publication in new window or tab >>Process design for production of Thai rosewood β-glucosidase in Pichia pastoris
2005 (English)Licentiate thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 2005. 50 p.
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-375 (URN)91-7178-081-5 (ISBN)
Presentation
2005-06-10, FB54, AlbaNova, Roslagstullsbacken 21, Stockholm, 11:00
Supervisors
Note
QC 20101126Available from: 2005-08-08 Created: 2005-08-08 Last updated: 2010-11-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Charoenrat, TheppanyaStendahl-Andersen, HelleJahic, MehmedalijaEnfors, Sven-Olof
By organisation
School of Biotechnology (BIO)Bioprocess Technology (closed 20130101)
In the same journal
Bioprocess and biosystems engineering (Print)
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 43 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf