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The active component in the flax-retting system of the zygomycete Rhizopus otyzae sb is a family 28 polygalacturonase
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Wood Chemistry and Pulp Technology.
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2005 (English)In: Journal of Industrial Microbiology & Biotechnology, ISSN 1367-5435, E-ISSN 1476-5535, Vol. 32, no 10, 431-438 p.Article in journal (Refereed) Published
Abstract [en]

The zygomycete Rhizopus oryzae sb is a very efficient organism for retting of flax, the initial microbiological step in the process of making linen. An extracellular polygalacturonase, when isolated could perform retting, and therefore probably is the key component in the retting system of R. oryzae. This was purified and characterized. The purified enzyme has a molecular mass of 37,436 Da from mass spectrometric determination, an isoelectric point of 8.4, and has non-methylated polygalacturonic acid as its preferred substrate. Peptide sequences indicate that the enzyme belongs to family 28, in similarity with other polygal-acturonases (EC. 3.2.1.15). It contains, however an N-terminal sequence absent in other fungal pectinases, but present in an enzyme from the phytopathogenic bacterium Ralstonia solanacearum. The biochemical background for the superior retting efficiency of R. oryzae sb is discussed.

Place, publisher, year, edition, pages
2005. Vol. 32, no 10, 431-438 p.
Keyword [en]
enzymatic retting, polygalacturonase, Rhizopus oryzae, pectin biodegradation, Ralstonia solanacearum, in-gel digestion, sequence-analysis, oxalic-acid, fungi, pectin, separation, mechanism, enzymes, oryzae, states
Identifiers
URN: urn:nbn:se:kth:diva-15190DOI: 10.1007/s10295-005-0014-yISI: 000233417100001Scopus ID: 2-s2.0-27444435985OAI: oai:DiVA.org:kth-15190DiVA: diva2:333231
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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CiteExportLink to record
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  • apa
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