Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi
KTH, School of Biotechnology (BIO), Environmental Microbiology.
2005 (English)In: Holzforschung, ISSN 0018-3830, E-ISSN 1437-434X, Vol. 59, no 6, 696-702 p.Article in journal (Refereed) Published
Abstract [en]

In the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation. The wood decay fungi Postia placenta and Coniophora puteana were detectable in interior wood samples by terminal restriction fragment length polymorphism (T-RFLP) after 2 weeks of incubation with monocultures, while microscopic detection of hyphae was not possible until after 7 weeks. A potential problem when fungal communities are studied with T-RFLPs of rDNA is that intra-specific variation complicates data analysis. In this work, we show that intra-specific sequence variation in the internal transcribed spacer of the rDNA in Coniophora puteana allows T-RFLP identification of this species. This is due to intra-specific variations in fragment length, in combination with the absence of point mutations in the selected restriction sites.

Place, publisher, year, edition, pages
2005. Vol. 59, no 6, 696-702 p.
Keyword [en]
brown rot, Coniophora, fungal colonisation, fungi, polymerase chain reaction (PCR), sequencing, terminal restriction fragment length polymorphism (T-RFLP), wood, serpula-lacrymans, molecular identity, ribosomal dna, rot fungi, polymorphism, communities, diversity, primers, rna
Identifiers
URN: urn:nbn:se:kth:diva-15228ISI: 000233697900016OAI: oai:DiVA.org:kth-15228DiVA: diva2:333269
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Land, Carl Johan
By organisation
Environmental Microbiology
In the same journal
Holzforschung

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf