Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
2005 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 10, no 5Article in journal (Refereed) Published
Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 X 2 fan-out diffractive optical elements (DOES), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 x 2 fiber arrays.
Place, publisher, year, edition, pages
2005. Vol. 10, no 5
cross-correlation, parallel, fluorescence correlation spectroscopy, single-molecule detection, fan-out element, gene-expression, statistical accuracy
IdentifiersURN: urn:nbn:se:kth:diva-15230ISI: 000233711300033OAI: oai:DiVA.org:kth-15230DiVA: diva2:333271
QC 201005252010-08-052010-08-05Bibliographically approved