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Serum microarrays for large scale screening of protein levels
KTH, School of Biotechnology (BIO), Molecular Biotechnology.
KTH, School of Biotechnology (BIO), Proteomics. KTH, School of Biotechnology (BIO), Molecular Biotechnology.
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2005 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 4, no 12, 1942-1947 p.Article in journal (Refereed) Published
Abstract [en]

There is a great need for comprehensive proteomic analysis of large patient cohorts of plasma and serum samples to identify biomarkers of human diseases. Here we describe a new antibody-based proteomic approach involving a reverse array format where serum samples are spotted on a microarray. This enables all samples to be screened for their content of a certain serum protein in a single experiment using target-recognizing antibodies and fluorescently labeled secondary antibodies. The procedure is illustrated with the analysis of the IgA levels in 2009 spotted serum samples, and the data are compared with clinical routine measurements. The results suggest that it is possible to simultaneously screen thousands of complex clinical serum samples for their content of the relative amount of specific serum proteins of clinical relevance.

Place, publisher, year, edition, pages
2005. Vol. 4, no 12, 1942-1947 p.
Keyword [en]
cancer, immunoassay, identification, specimens, arrays
URN: urn:nbn:se:kth:diva-15236DOI: 10.1074/mcp.M500213-MCP200ISI: 000233774200010ScopusID: 2-s2.0-29244474075OAI: diva2:333277
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2011-02-09Bibliographically approved

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Ödling, JennySundberg, MårtenLundeberg, JoakimUhlén, MathiasNilsson, Peter
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