Cytogenetic and expression profiles associated with transformation to androgen-resistant prostate cancer
2006 (English)In: The Prostate, ISSN 0270-4137, E-ISSN 1097-0045, Vol. 66, no 2, 157-172 p.Article in journal (Refereed) Published
BACKGROUND. The mechanisms underlying the progression of prostate cancer to androgen-resistant cancer are still not fully understood. Here, we studied the genetic events associated with this transformation. METHODS. The androgen sensitive prostate cancer cells line LNCaP-FGC and its androgen resistant subline LNCaP-r were investigated using SKY, CGH, and cDNA microarray. RESULTS. Karyotypically, several additional chromosomal aberrations were seen in LNCaP-r as compared to the parental line. CGH also revealed unique net chromosomal alterations in LNCaP-r compared to LNCaP-FGC, including gain of 2p13-23, 2q21-32, and 13q and loss of 6p22-pter. cDNA microarray analysis identified several genes involved in DNA methylation, such as DNMT2, DNMT3a, and methyl-CpG binding domain protein 2 and 4 that were higher expressed in LNCaP-r. Interestingly, androgen responsiveness of LNCaP-r was restored after treated with DNA methyltransferase inhibitor. CONCLUSIONS. Our findings may serve as a basis for molecular dissection of the mechanisms involved in development of androgen resistant prostate cancer.
Place, publisher, year, edition, pages
2006. Vol. 66, no 2, 157-172 p.
prostate cancer, androgen resistance, DNA microarray, spectral karyotype, CGH, comparative genomic hybridization, in-situ hybridization, cell-lines, genetic alterations, lncap-r, methylation, carcinoma, progression, proteins, family
IdentifiersURN: urn:nbn:se:kth:diva-15386DOI: 10.1002/pros.20328ISI: 000234838300005ScopusID: 2-s2.0-31944447676OAI: oai:DiVA.org:kth-15386DiVA: diva2:333427
QC 201005252010-08-052010-08-05Bibliographically approved