Adhesion forces between protein layers studied by means of atomic force microscopy
2006 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 22, no 11, 5108-5114 p.Article in journal (Refereed) Published
Adhesion forces between different protein layers adsorbed on different substrates in aqueous media have been measured by means of an atomic force microscope using the colloid probe technique. The effects of the loading force, the salt concentration and pH of the medium, and the electrolyte type on the strength, the pull-off distance, and the separation energy of such adhesion forces have been analyzed in depth. Two very different proteins (bovine serum albumin and apoferritin) and two dissimilar substrates (silica and polystyrene) were used in the experiments. The results clearly point out a very important contribution of the electrostatic interactions in the adhesion between protein layers.
Place, publisher, year, edition, pages
2006. Vol. 22, no 11, 5108-5114 p.
bovine serum-albumin, streptococcus-mitis strains, bacterial adhesion, silica surfaces, colloidal stability, hydration forces, apoferritin, adsorption, model, bsa
IdentifiersURN: urn:nbn:se:kth:diva-15679DOI: 10.1021/la053011kISI: 000237593000036ScopusID: 2-s2.0-33745746027OAI: oai:DiVA.org:kth-15679DiVA: diva2:333721
QC 201005252010-08-052010-08-05Bibliographically approved