Magnetic resonance tracking of nanoparticle labelled neural stem cells in a rat's spinal cord
2006 (English)In: Nanotechnology, ISSN 0957-4484, E-ISSN 1361-6528, Vol. 17, no 8, 1911-1915 p.Article in journal (Refereed) Published
Neural stem cells isolated from an adult rat's spinal cord were loaded with superparamagnetic gold-coated monocrystalline iron oxide nanoparticles (Au-MION) intended for use as contrast enhancers in magnetic resonance imaging (MRI). A dose-dependent attenuation of MRI signals was observed for Au-MION down to 0.001 mu gFe/mu l and for nanoparticle-loaded clusters of only 20 cells. The labelled cells were infused into the spinal cord of anaesthetized rats and tracked by MRI at 1 h, 48 h and 1 month post-injection. Histological analysis revealed that MRI signals correlated well with gold-positive staining of transplanted cells. The present results show that Au-MION exerts powerful contrast-enhancing properties and may represent novel MRI labels for labelling and tracking the transplanted cells in vivo.
Place, publisher, year, edition, pages
2006. Vol. 17, no 8, 1911-1915 p.
superparamagnetic iron-oxide, in-vivo tracking, contrast agents, radicals
IdentifiersURN: urn:nbn:se:kth:diva-15701DOI: 10.1088/0957-4484/17/8/018ISI: 000237813000019ScopusID: 2-s2.0-33645321436OAI: oai:DiVA.org:kth-15701DiVA: diva2:333743
QC 201005252010-08-052010-08-05Bibliographically approved