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A human protein atlas based on antibody proteomics
KTH, School of Biotechnology (BIO), Proteomics.
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0003-0605-8417
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-8993-048X
2006 (English)In: Current opinion in molecular therapeutics (Print), ISSN 1464-8431, E-ISSN 2040-3445, Vol. 8, no 3, 185-190 p.Article in journal (Refereed) Published
Abstract [en]

The Human Protein Atlas is a comprehensive database that provides the protein expression profiles for a large number of human proteins, presented as immunohistological images from most human tissues. This review provides an overview of the contents of the atlas, discusses the project strategy and highlights the importance of open access for data validation and quality. Essential procedures that are implemented during antibody production and image generation, such as the use of protein epitope signature tags ( PrEST) antigens, monospecific antibodies, tissue microarrays and thorough quality validation, are also discussed. The Human Protein Atlas is related to four other expression atlas initiatives, including, in particular, an upcoming protein atlas developed by the Sanger Institute.

Place, publisher, year, edition, pages
2006. Vol. 8, no 3, 185-190 p.
Keyword [en]
immunohistochemistry, monospecific antibody, PrEST, protein atlas, protein expression, tissue microarray, tissue microarrays, gene-expression, human genome, tool, generation, products, database, project
Identifiers
URN: urn:nbn:se:kth:diva-15718ISI: 000237946300002Scopus ID: 2-s2.0-33744922423OAI: oai:DiVA.org:kth-15718DiVA: diva2:333760
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved

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Hober, SophiaUhlén, Mathias

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